Abstract
Although chemopreventive anti‐steroids such as the antiestrogens are thought to act through competitive inhibition of agonist binding to estrogen receptors, it has been postulated that the estrogen receptor changes its conformation when bound to a strong antiestrogen such as ICI‐164,384. We hypothesized that such conformationally changed receptors could bind specific recognition sequences in the genome and activate specific genes that might be involved in growth arrest. In order to identify such genes with a functional assay, we used a retroviral gene trap U3lacZ. We have now isolated MCF‐7 breast cancer cell line clones in which the lacZ reporter gene is inserted into the genes activated by either ICI‐164,384 or retinoic acid. One such clone, B4, was further characterized. In B4, lacZ activity is induced by ICI‐164,384 or trans‐retinoic acid, and repressed after treatment with estradiol. Cloning of the 5′‐flanking genomic sequence in this clone will be possible using polymerase chain reaction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 254 |
| Number of pages | 1 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 53 |
| Issue number | S17G |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Trapping of genes induced upon growth arrest after treatment with antiestrogen or retinoids using retroviral promoter trap. / Paik, Soonmyoung; Zwiebel, James A.; Lippman, Marc E.
In: Journal of Cellular Biochemistry, Vol. 53, No. S17G, 1993, p. 254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Trapping of genes induced upon growth arrest after treatment with antiestrogen or retinoids using retroviral promoter trap
AU - Paik, Soonmyoung
AU - Zwiebel, James A.
AU - Lippman, Marc E
PY - 1993
Y1 - 1993
N2 - Although chemopreventive anti‐steroids such as the antiestrogens are thought to act through competitive inhibition of agonist binding to estrogen receptors, it has been postulated that the estrogen receptor changes its conformation when bound to a strong antiestrogen such as ICI‐164,384. We hypothesized that such conformationally changed receptors could bind specific recognition sequences in the genome and activate specific genes that might be involved in growth arrest. In order to identify such genes with a functional assay, we used a retroviral gene trap U3lacZ. We have now isolated MCF‐7 breast cancer cell line clones in which the lacZ reporter gene is inserted into the genes activated by either ICI‐164,384 or retinoic acid. One such clone, B4, was further characterized. In B4, lacZ activity is induced by ICI‐164,384 or trans‐retinoic acid, and repressed after treatment with estradiol. Cloning of the 5′‐flanking genomic sequence in this clone will be possible using polymerase chain reaction.
AB - Although chemopreventive anti‐steroids such as the antiestrogens are thought to act through competitive inhibition of agonist binding to estrogen receptors, it has been postulated that the estrogen receptor changes its conformation when bound to a strong antiestrogen such as ICI‐164,384. We hypothesized that such conformationally changed receptors could bind specific recognition sequences in the genome and activate specific genes that might be involved in growth arrest. In order to identify such genes with a functional assay, we used a retroviral gene trap U3lacZ. We have now isolated MCF‐7 breast cancer cell line clones in which the lacZ reporter gene is inserted into the genes activated by either ICI‐164,384 or retinoic acid. One such clone, B4, was further characterized. In B4, lacZ activity is induced by ICI‐164,384 or trans‐retinoic acid, and repressed after treatment with estradiol. Cloning of the 5′‐flanking genomic sequence in this clone will be possible using polymerase chain reaction.
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UR - http://www.scopus.com/inward/citedby.url?scp=84991195188&partnerID=8YFLogxK
U2 - 10.1002/jcb.240531155
DO - 10.1002/jcb.240531155
M3 - Article
VL - 53
SP - 254
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - S17G
ER -