Transscleral coulomb controlled iontophoresis of ganciclovir in rabbits: Safety and pharmacokinetics

S. Gautier, L. Kasner, F. Behsr-Cohen, A. El Aouni, H. Kondo, G. O'Grady, J. L. Davis, J. M. Parsl

Research output: Contribution to journalArticle

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Abstract

Purpose. To demonstrate safety of transscleral Coulomb Controlled Iontophoresis (CCI) and to détermine ocular pharmocokinetics of ganciclovir after iontophoretic treatment in rabbits Methods. 4 pilot studies were carried out using 3 transscleral electrodes and 3 current intensities. A total of 37 eyes received CCI with an aqueous ganciclovir sodium solution of 20%. Intravitreal injection of 2000 jg (0.1 ml) was performed as a control in 18 eyes. Safety: Histology was p?rformed in 2 eyes after CCI with 500 uA (OS) and up to 4000 uA (OD) for IO min (at 72 hrs). Pharmacokinetics: CCI was performed with 500 and 2500 uA for 10 min. Ganciclovir concentration was determined by HF'LC for all ocular fluids and tissues at 2, 24, 72 and 168 hrs and at 1, 8 and 24 hrs after treatment. Results. Histology revealed no lesions, burn or damage in any tissue, except for an inflammatory response in the conjunctiva of OD Therapeutic mtravitreal levels were found and the mechanii.m of ganciclovir ocular distribution is discussed. Conclusion. CCI was ssfe and effective to deliver ganciclovir into the posterior segment of the rabbit eye without causing any lesion. It is a promising alternative treatment of C MV retinitis.

Original languageEnglish (US)
Pages (from-to)S147
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Gautier, S., Kasner, L., Behsr-Cohen, F., El Aouni, A., Kondo, H., O'Grady, G., Davis, J. L., & Parsl, J. M. (1997). Transscleral coulomb controlled iontophoresis of ganciclovir in rabbits: Safety and pharmacokinetics. Investigative Ophthalmology and Visual Science, 38(4), S147.