Transplanted neurons integrate into adult retinas and respond to light

Praseeda Venugopalan, Yan Wang, Tu Nguyen, Abigail Huang, Kenneth J Muller, Jeffrey L. Goldberg

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Retinal ganglion cells (RGCs) degenerate in diseases like glaucoma and are not replaced in adult mammals. Here we investigate whether transplanted RGCs can integrate into the mature retina. We have transplanted GFP-labelled RGCs into uninjured rat retinas in vivo by intravitreal injection. Transplanted RGCs acquire the general morphology of endogenous RGCs, with axons orienting towards the optic nerve head of the host retina and dendrites growing into the inner plexiform layer. Preliminary data show in some cases GFP+ axons extending within the host optic nerves and optic tract, reaching usual synaptic targets in the brain, including the lateral geniculate nucleus and superior colliculus. Electrophysiological recordings from transplanted RGCs demonstrate the cells' electrical excitability and light responses similar to host ON, ON-OFF and OFF RGCs, although less rapid and with greater adaptation. These data present a promising approach to develop cell replacement strategies in diseased retinas with degenerating RGCs.

Original languageEnglish (US)
Article number10472
JournalNature Communications
Volume7
DOIs
StatePublished - Feb 4 2016

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Retinal Ganglion Cells
retina
neurons
Neurons
Retina
Optics
Light
cells
Mammals
Rats
Brain
axons
Axons
nerves
optics
Geniculate Bodies
Intravitreal Injections
Superior Colliculi
glaucoma
Optic Disk

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Transplanted neurons integrate into adult retinas and respond to light. / Venugopalan, Praseeda; Wang, Yan; Nguyen, Tu; Huang, Abigail; Muller, Kenneth J; Goldberg, Jeffrey L.

In: Nature Communications, Vol. 7, 10472, 04.02.2016.

Research output: Contribution to journalArticle

Venugopalan, Praseeda ; Wang, Yan ; Nguyen, Tu ; Huang, Abigail ; Muller, Kenneth J ; Goldberg, Jeffrey L. / Transplanted neurons integrate into adult retinas and respond to light. In: Nature Communications. 2016 ; Vol. 7.
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