Transplanted neural progenitor cells expressing mutant NT3 promote myelination and partial hindlimb recovery in the chronic phase after spinal cord injury

Kazuo Kusano, Mitsuhiro Enomoto, Takashi Hirai, Pantelis Tsoulfas, Shinichi Sotome, Kenichi Shinomiya, Atsushi Okawa

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Neutrotrophin-3 (NT3) plays a protective role in injured central nervous system tissues through interaction with trk receptors. To enhance the regeneration of damaged tissue, a combination therapy with cell transplantation and neurotrophins has been under development. We examined whether the transplantation of neural progenitor cells (NPCs) secreting NT3/D15A, a multi-neurotrophin with the capacity to bind both trkB and trkC, would enhance the repair of damaged tissues and the functional recovery in a chronic phase of spinal cord injury. The cultured NPCs with lentiviral vector containing either GFP or NT3/D15A were transplanted into the contused spinal cord at 6 weeks after the initial thoracic injury. Eight weeks after the transplantation, the NT3/D15A transplants displayed better survival than the GFP transplants, and they exhibited enhanced myelin formation and partial improvement of hindlimb function. Our study revealed that NT3/D15A produced positive effects in injured spinal cords even in the chronic phase. These effects suggest an enhanced neurotrophin-trk signaling by NT3/D15A.

Original languageEnglish (US)
Pages (from-to)812-817
Number of pages6
JournalBiochemical and biophysical research communications
Volume393
Issue number4
DOIs
StatePublished - Mar 19 2010

Keywords

  • Chronic phase
  • Neural progenitor cells
  • Neurotrophins
  • Spinal cord injury
  • Transplantation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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