Transplantation of cryopreserved human pancreatic islets into diabetic nude mice

Camillo Ricordi, Norman M. Kneteman, David W. Scharp, Paul E. Lacy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

If pancreatic islet transplantation becomes clinically applicable, cryogenic storage of human islet preparations could solve many problems related to transportation and banking of islets, HLA matching, recipient pretreatment, or the possible use of multiple donors. The diabetic nude mouse could offer a simple model to test in vivo the function of cryopreserved human islet preparations before transplantation into diabetic patients. In this study, 6 nude mice made diabetic by an intravenous injection of streptozocin were transplanted with 400-600 cryopreserved human islets beneath the renal capsule. All the mice became normoglycemic within 3 weeks from transplantation. Nephrectomy of the kidneys bearing the grafts 45 days after transplantation resulted in an immediate return to the diabetic state, demonstrating that the only functional tissue was located in the excised kidneys. Histologic study of the renal subcapsular grafts demonstrated morphologic integrity of the islets with a normal degree of beta granulation. The results demonstrated that cryopreserved human islets will function in diabetic nude mice. This model to test human islet preparationsin vivomay be of assistance in the application of cryopreserved islet transplantation in diabetic patients.

Original languageEnglish
Pages (from-to)861-864
Number of pages4
JournalWorld Journal of Surgery
Volume12
Issue number6
DOIs
StatePublished - Dec 1 1988
Externally publishedYes

Fingerprint

Islets of Langerhans
Nude Mice
Transplantation
Kidney
Islets of Langerhans Transplantation
Transplants
Streptozocin
Nephrectomy
Intravenous Injections
Capsules
Tissue Donors

ASJC Scopus subject areas

  • Surgery

Cite this

Transplantation of cryopreserved human pancreatic islets into diabetic nude mice. / Ricordi, Camillo; Kneteman, Norman M.; Scharp, David W.; Lacy, Paul E.

In: World Journal of Surgery, Vol. 12, No. 6, 01.12.1988, p. 861-864.

Research output: Contribution to journalArticle

Ricordi, Camillo ; Kneteman, Norman M. ; Scharp, David W. ; Lacy, Paul E. / Transplantation of cryopreserved human pancreatic islets into diabetic nude mice. In: World Journal of Surgery. 1988 ; Vol. 12, No. 6. pp. 861-864.
@article{99bd1573f71840ba8550a6df20c695da,
title = "Transplantation of cryopreserved human pancreatic islets into diabetic nude mice",
abstract = "If pancreatic islet transplantation becomes clinically applicable, cryogenic storage of human islet preparations could solve many problems related to transportation and banking of islets, HLA matching, recipient pretreatment, or the possible use of multiple donors. The diabetic nude mouse could offer a simple model to test in vivo the function of cryopreserved human islet preparations before transplantation into diabetic patients. In this study, 6 nude mice made diabetic by an intravenous injection of streptozocin were transplanted with 400-600 cryopreserved human islets beneath the renal capsule. All the mice became normoglycemic within 3 weeks from transplantation. Nephrectomy of the kidneys bearing the grafts 45 days after transplantation resulted in an immediate return to the diabetic state, demonstrating that the only functional tissue was located in the excised kidneys. Histologic study of the renal subcapsular grafts demonstrated morphologic integrity of the islets with a normal degree of beta granulation. The results demonstrated that cryopreserved human islets will function in diabetic nude mice. This model to test human islet preparationsin vivomay be of assistance in the application of cryopreserved islet transplantation in diabetic patients.",
author = "Camillo Ricordi and Kneteman, {Norman M.} and Scharp, {David W.} and Lacy, {Paul E.}",
year = "1988",
month = "12",
day = "1",
doi = "10.1007/BF01655500",
language = "English",
volume = "12",
pages = "861--864",
journal = "World Journal of Surgery",
issn = "0364-2313",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Transplantation of cryopreserved human pancreatic islets into diabetic nude mice

AU - Ricordi, Camillo

AU - Kneteman, Norman M.

AU - Scharp, David W.

AU - Lacy, Paul E.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - If pancreatic islet transplantation becomes clinically applicable, cryogenic storage of human islet preparations could solve many problems related to transportation and banking of islets, HLA matching, recipient pretreatment, or the possible use of multiple donors. The diabetic nude mouse could offer a simple model to test in vivo the function of cryopreserved human islet preparations before transplantation into diabetic patients. In this study, 6 nude mice made diabetic by an intravenous injection of streptozocin were transplanted with 400-600 cryopreserved human islets beneath the renal capsule. All the mice became normoglycemic within 3 weeks from transplantation. Nephrectomy of the kidneys bearing the grafts 45 days after transplantation resulted in an immediate return to the diabetic state, demonstrating that the only functional tissue was located in the excised kidneys. Histologic study of the renal subcapsular grafts demonstrated morphologic integrity of the islets with a normal degree of beta granulation. The results demonstrated that cryopreserved human islets will function in diabetic nude mice. This model to test human islet preparationsin vivomay be of assistance in the application of cryopreserved islet transplantation in diabetic patients.

AB - If pancreatic islet transplantation becomes clinically applicable, cryogenic storage of human islet preparations could solve many problems related to transportation and banking of islets, HLA matching, recipient pretreatment, or the possible use of multiple donors. The diabetic nude mouse could offer a simple model to test in vivo the function of cryopreserved human islet preparations before transplantation into diabetic patients. In this study, 6 nude mice made diabetic by an intravenous injection of streptozocin were transplanted with 400-600 cryopreserved human islets beneath the renal capsule. All the mice became normoglycemic within 3 weeks from transplantation. Nephrectomy of the kidneys bearing the grafts 45 days after transplantation resulted in an immediate return to the diabetic state, demonstrating that the only functional tissue was located in the excised kidneys. Histologic study of the renal subcapsular grafts demonstrated morphologic integrity of the islets with a normal degree of beta granulation. The results demonstrated that cryopreserved human islets will function in diabetic nude mice. This model to test human islet preparationsin vivomay be of assistance in the application of cryopreserved islet transplantation in diabetic patients.

UR - http://www.scopus.com/inward/record.url?scp=0024246428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024246428&partnerID=8YFLogxK

U2 - 10.1007/BF01655500

DO - 10.1007/BF01655500

M3 - Article

C2 - 3150638

AN - SCOPUS:0024246428

VL - 12

SP - 861

EP - 864

JO - World Journal of Surgery

JF - World Journal of Surgery

SN - 0364-2313

IS - 6

ER -