Transmission light microscopy of structurally colored semithin cartilage sections

G. I. Malinin; H. K. Lo; T. I. Malinin

doi:10.1007/s004180050196

Transmission light microscopy of structurally colored semithin cartilage sections

G. I. Malinin, H. K. Lo, T. I. Malinin

Orthopaedics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Conversion of osmified tracheal cartilage constituents into an array of laminar interference gratings has been attained by three tandem reactions. Oxidation of semithin, LR white-embedded cartilage sections by acetic anhydride in dimethyl sulfoxide is the first step in the conversion process. Subsequent addition reactions of oxidized cartilage pyranoses and furanoses with thiocarbohydrazide constitutes the second step. Reduction of silver proteinate by thiocarbohydrazones and the concomitant coating of cartilage constituents with silver gratings completes the conversion of cartilage sections into a system of layered interference filters. In transmitted light, all components of converted cartilage display vivid structural colors, which allow detailed microscopic analysis of structurally colored cellular and extracellular cartilage constituents.

Original language	English (US)
Pages (from-to)	1-10
Number of pages	10
Journal	Histochemistry and Cell Biology
Volume	109
Issue number	1
DOIs	https://doi.org/10.1007/s004180050196
State	Published - Dec 1997

ASJC Scopus subject areas

Histology
Molecular Biology
Medical Laboratory Technology
Cell Biology

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author = "Malinin, {G. I.} and Lo, {H. K.} and Malinin, {T. I.}",

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AU - Lo, H. K.

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AB - Conversion of osmified tracheal cartilage constituents into an array of laminar interference gratings has been attained by three tandem reactions. Oxidation of semithin, LR white-embedded cartilage sections by acetic anhydride in dimethyl sulfoxide is the first step in the conversion process. Subsequent addition reactions of oxidized cartilage pyranoses and furanoses with thiocarbohydrazide constitutes the second step. Reduction of silver proteinate by thiocarbohydrazones and the concomitant coating of cartilage constituents with silver gratings completes the conversion of cartilage sections into a system of layered interference filters. In transmitted light, all components of converted cartilage display vivid structural colors, which allow detailed microscopic analysis of structurally colored cellular and extracellular cartilage constituents.

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Transmission light microscopy of structurally colored semithin cartilage sections

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