Transmission light microscopy of structurally colored semithin cartilage sections

G. I. Malinin, H. K. Lo, T. I. Malinin

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Conversion of osmified tracheal cartilage constituents into an array of laminar interference gratings has been attained by three tandem reactions. Oxidation of semithin, LR white-embedded cartilage sections by acetic anhydride in dimethyl sulfoxide is the first step in the conversion process. Subsequent addition reactions of oxidized cartilage pyranoses and furanoses with thiocarbohydrazide constitutes the second step. Reduction of silver proteinate by thiocarbohydrazones and the concomitant coating of cartilage constituents with silver gratings completes the conversion of cartilage sections into a system of layered interference filters. In transmitted light, all components of converted cartilage display vivid structural colors, which allow detailed microscopic analysis of structurally colored cellular and extracellular cartilage constituents.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalHistochemistry and Cell Biology
Volume109
Issue number1
DOIs
StatePublished - Dec 1997

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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