Transmembrane movement of the Shaker K+ channel S4

Hans P Larsson; Oliver S. Baker; Dalvinder S. Dhillon; Ehud Y. Isacoff

doi:10.1016/S0896-6273(00)80056-2

Transmembrane movement of the Shaker K⁺ channel S4

Hans P Larsson, Oliver S. Baker, Dalvinder S. Dhillon, Ehud Y. Isacoff

Research output: Contribution to journal › Article

430 Citations (Scopus)

Abstract

We have probed internal and external accessibility of S4 residues to the membrane-impermeant thiol reagent methanethiosulfonate- ethyltrimethylammonium (MTSET) in both open and closed, cysteine-substituted Shaker K⁺ channels. Our results indicate that S4 traverses the membrane with no more than 5 amino acids in the closed state, and that the distribution of buried residues changes when channels open. This change argues for a displacement of S4 through the plane of the membrane in which an initially intracellular residue moves to within 3 amino acids of the extracellular solution. These results demonstrate that the putative voltage-sensing charges of S4 actually reside in the membrane and that they move outward when channels open. We consider constraints placed on channel structure by these results.

Original language	English
Pages (from-to)	387-397
Number of pages	11
Journal	Neuron
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/S0896-6273(00)80056-2
State	Published - Feb 1 1996
Externally published	Yes

Fingerprint

Membranes

Amino Acids

Sulfhydryl Reagents

Cysteine

trimethylethylammonium

methanethiosulfonate

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Larsson, H. P., Baker, O. S., Dhillon, D. S., & Isacoff, E. Y. (1996). Transmembrane movement of the Shaker K⁺ channel S4. Neuron, 16(2), 387-397. https://doi.org/10.1016/S0896-6273(00)80056-2

Transmembrane movement of the Shaker K⁺ channel S4. / Larsson, Hans P; Baker, Oliver S.; Dhillon, Dalvinder S.; Isacoff, Ehud Y.

In: Neuron, Vol. 16, No. 2, 01.02.1996, p. 387-397.

Research output: Contribution to journal › Article

Larsson, HP, Baker, OS, Dhillon, DS & Isacoff, EY 1996, 'Transmembrane movement of the Shaker K⁺ channel S4', Neuron, vol. 16, no. 2, pp. 387-397. https://doi.org/10.1016/S0896-6273(00)80056-2

Larsson HP, Baker OS, Dhillon DS, Isacoff EY. Transmembrane movement of the Shaker K⁺ channel S4. Neuron. 1996 Feb 1;16(2):387-397. https://doi.org/10.1016/S0896-6273(00)80056-2

Larsson, Hans P ; Baker, Oliver S. ; Dhillon, Dalvinder S. ; Isacoff, Ehud Y. / Transmembrane movement of the Shaker K⁺ channel S4. In: Neuron. 1996 ; Vol. 16, No. 2. pp. 387-397.

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10.1016/S0896-6273(00)80056-2