Transitional type 1 and 2 B lymphocyte subsets are differentially responsive to antigen receptor signaling

James B. Petro, Rachel M. Gerstein, John Lowe, Robert S. Carter, Nicholas Shinners, Wasif Khan

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Mature B-lymphocytes develop sequentially from transitional type 1 (TI) and type 2 (T2) precursors in the spleen. To elucidate the mechanisms that regulate the developmental fate of these distinct B cell subsets, we investigated their biochemical and biological responses following stimulation through the B-cell antigen receptor (BCR). As compared with the TI subset, T2 cells are more responsive to BCR engagement, as evidenced by their rohust induction of activation markers, expression of the prosurvival protein Bcl-xL, and enhanced proliferation. BCR stimulation of T2 cells leads to the appearance of B cells with mature phenotypic characteristics, whereas T1 cells die. All of these T2 responses are dependent on the BCR signal transducer Bruton's tyrosine kinase, which is dispensable for the T1 to T2 transition. Furthermore, the serine/threonine kinases ERK, p38 MAPK, and Akt are predominantly activated in T2 compared with T1 B cells following BCR cross-linking. We conclude that T1 and T2 B cells respond differentially to BCR engagement via the induction of stage-specific signaling pathways. In turn, these signaling pathways probably govern the development and selection processes that are critical for the formation of the mature B cell compartment.

Original languageEnglish
Pages (from-to)48009-48019
Number of pages11
JournalJournal of Biological Chemistry
Volume277
Issue number50
DOIs
StatePublished - Dec 13 2002
Externally publishedYes

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B-Lymphocyte Subsets
B-Cell Antigen Receptors
Antigen Receptors
Lymphocytes
B-Lymphocytes
Cells
Protein-Serine-Threonine Kinases
p38 Mitogen-Activated Protein Kinases
Transducers
Spleen
Chemical activation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transitional type 1 and 2 B lymphocyte subsets are differentially responsive to antigen receptor signaling. / Petro, James B.; Gerstein, Rachel M.; Lowe, John; Carter, Robert S.; Shinners, Nicholas; Khan, Wasif.

In: Journal of Biological Chemistry, Vol. 277, No. 50, 13.12.2002, p. 48009-48019.

Research output: Contribution to journalArticle

Petro, James B. ; Gerstein, Rachel M. ; Lowe, John ; Carter, Robert S. ; Shinners, Nicholas ; Khan, Wasif. / Transitional type 1 and 2 B lymphocyte subsets are differentially responsive to antigen receptor signaling. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 50. pp. 48009-48019.
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