Transient expression of stage-specific embryonic antigen-1 (CD15) in the developing dorsal rat spinal cord

Martin Oudega, Enrico Marani, Rath T W M Thomeer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The localization of CD15 (synonyms: stage-specific embryonic antigen-1 (SSEA-1), 3(α)-fucosyl-N-acetyl-lactosamine or FAL), which is implicated in neuronal differentiation, in the developing dorsal rat spinal cord was studied by immunocytochemistry. A embryonal day 9 (E9), SSEA-1 was detected in the neural ectoderm and, at E11, in cells near the ventricle of the matrix layer. This localization indicated that SSEA-1 is present in proliferating premigratory cells of the rat spinal cord. Between E12 and E16, cells of the alar plate expressed SSEA-1. Expression of the antigen was restricted to neuroblasts that will form the dorsal horn. SSEA-1, therefore, can be used at this stage as a marker for a subdivision of the matrix layer. At E14, the dorsal root entrance zone showed SSEA-1. This indicated that SSEA-1 is associated with ingrowing primary afferents. From E16 on, SSEA-1 was present in the dorsal raphe, which suggested a function for SSEA-1 in the guidance of developing fibres. After E17, the antigen was also found within the dorsal mantle layer. SSEA-1 was first present in Rexed's laminae II, IV and V. Later on in development the antigen was detected only in Rexed's laminae II (substantia gelatinosa). These distribution patterns indicated that SSEA-1 is present on migratory and/or postmigratory cells. In addition, SSEA-1 is associated with small-diameter dorsal root fibres, the C fibres and A(∂) fibres, that terminate within the substantia gelatinosa. After birth SSEA-1 was present throughout the dorsal horn, probably as a result of the myelination of the fibres.

Original languageEnglish (US)
Pages (from-to)869-877
Number of pages9
JournalThe Histochemical Journal
Volume24
Issue number11
DOIs
StatePublished - Nov 1992
Externally publishedYes

Fingerprint

CD15 Antigens
Spinal Cord
Substantia Gelatinosa
Spinal Nerve Roots
Antigens
Myelinated Nerve Fibers
Unmyelinated Nerve Fibers
Ectoderm

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Transient expression of stage-specific embryonic antigen-1 (CD15) in the developing dorsal rat spinal cord. / Oudega, Martin; Marani, Enrico; Thomeer, Rath T W M.

In: The Histochemical Journal, Vol. 24, No. 11, 11.1992, p. 869-877.

Research output: Contribution to journalArticle

@article{3148c93a4bed409cad144f27c40f0f6f,
title = "Transient expression of stage-specific embryonic antigen-1 (CD15) in the developing dorsal rat spinal cord",
abstract = "The localization of CD15 (synonyms: stage-specific embryonic antigen-1 (SSEA-1), 3(α)-fucosyl-N-acetyl-lactosamine or FAL), which is implicated in neuronal differentiation, in the developing dorsal rat spinal cord was studied by immunocytochemistry. A embryonal day 9 (E9), SSEA-1 was detected in the neural ectoderm and, at E11, in cells near the ventricle of the matrix layer. This localization indicated that SSEA-1 is present in proliferating premigratory cells of the rat spinal cord. Between E12 and E16, cells of the alar plate expressed SSEA-1. Expression of the antigen was restricted to neuroblasts that will form the dorsal horn. SSEA-1, therefore, can be used at this stage as a marker for a subdivision of the matrix layer. At E14, the dorsal root entrance zone showed SSEA-1. This indicated that SSEA-1 is associated with ingrowing primary afferents. From E16 on, SSEA-1 was present in the dorsal raphe, which suggested a function for SSEA-1 in the guidance of developing fibres. After E17, the antigen was also found within the dorsal mantle layer. SSEA-1 was first present in Rexed's laminae II, IV and V. Later on in development the antigen was detected only in Rexed's laminae II (substantia gelatinosa). These distribution patterns indicated that SSEA-1 is present on migratory and/or postmigratory cells. In addition, SSEA-1 is associated with small-diameter dorsal root fibres, the C fibres and A(∂) fibres, that terminate within the substantia gelatinosa. After birth SSEA-1 was present throughout the dorsal horn, probably as a result of the myelination of the fibres.",
author = "Martin Oudega and Enrico Marani and Thomeer, {Rath T W M}",
year = "1992",
month = "11",
doi = "10.1007/BF01046358",
language = "English (US)",
volume = "24",
pages = "869--877",
journal = "Journal of Molecular Histology",
issn = "1567-2379",
publisher = "Springer Netherlands",
number = "11",

}

TY - JOUR

T1 - Transient expression of stage-specific embryonic antigen-1 (CD15) in the developing dorsal rat spinal cord

AU - Oudega, Martin

AU - Marani, Enrico

AU - Thomeer, Rath T W M

PY - 1992/11

Y1 - 1992/11

N2 - The localization of CD15 (synonyms: stage-specific embryonic antigen-1 (SSEA-1), 3(α)-fucosyl-N-acetyl-lactosamine or FAL), which is implicated in neuronal differentiation, in the developing dorsal rat spinal cord was studied by immunocytochemistry. A embryonal day 9 (E9), SSEA-1 was detected in the neural ectoderm and, at E11, in cells near the ventricle of the matrix layer. This localization indicated that SSEA-1 is present in proliferating premigratory cells of the rat spinal cord. Between E12 and E16, cells of the alar plate expressed SSEA-1. Expression of the antigen was restricted to neuroblasts that will form the dorsal horn. SSEA-1, therefore, can be used at this stage as a marker for a subdivision of the matrix layer. At E14, the dorsal root entrance zone showed SSEA-1. This indicated that SSEA-1 is associated with ingrowing primary afferents. From E16 on, SSEA-1 was present in the dorsal raphe, which suggested a function for SSEA-1 in the guidance of developing fibres. After E17, the antigen was also found within the dorsal mantle layer. SSEA-1 was first present in Rexed's laminae II, IV and V. Later on in development the antigen was detected only in Rexed's laminae II (substantia gelatinosa). These distribution patterns indicated that SSEA-1 is present on migratory and/or postmigratory cells. In addition, SSEA-1 is associated with small-diameter dorsal root fibres, the C fibres and A(∂) fibres, that terminate within the substantia gelatinosa. After birth SSEA-1 was present throughout the dorsal horn, probably as a result of the myelination of the fibres.

AB - The localization of CD15 (synonyms: stage-specific embryonic antigen-1 (SSEA-1), 3(α)-fucosyl-N-acetyl-lactosamine or FAL), which is implicated in neuronal differentiation, in the developing dorsal rat spinal cord was studied by immunocytochemistry. A embryonal day 9 (E9), SSEA-1 was detected in the neural ectoderm and, at E11, in cells near the ventricle of the matrix layer. This localization indicated that SSEA-1 is present in proliferating premigratory cells of the rat spinal cord. Between E12 and E16, cells of the alar plate expressed SSEA-1. Expression of the antigen was restricted to neuroblasts that will form the dorsal horn. SSEA-1, therefore, can be used at this stage as a marker for a subdivision of the matrix layer. At E14, the dorsal root entrance zone showed SSEA-1. This indicated that SSEA-1 is associated with ingrowing primary afferents. From E16 on, SSEA-1 was present in the dorsal raphe, which suggested a function for SSEA-1 in the guidance of developing fibres. After E17, the antigen was also found within the dorsal mantle layer. SSEA-1 was first present in Rexed's laminae II, IV and V. Later on in development the antigen was detected only in Rexed's laminae II (substantia gelatinosa). These distribution patterns indicated that SSEA-1 is present on migratory and/or postmigratory cells. In addition, SSEA-1 is associated with small-diameter dorsal root fibres, the C fibres and A(∂) fibres, that terminate within the substantia gelatinosa. After birth SSEA-1 was present throughout the dorsal horn, probably as a result of the myelination of the fibres.

UR - http://www.scopus.com/inward/record.url?scp=0026498275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026498275&partnerID=8YFLogxK

U2 - 10.1007/BF01046358

DO - 10.1007/BF01046358

M3 - Article

C2 - 1362198

AN - SCOPUS:0026498275

VL - 24

SP - 869

EP - 877

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

SN - 1567-2379

IS - 11

ER -