Transient enhanced IL-2R signaling early during priming rapidly amplifies development of functional CD8+ T effector-memory cells

Iris Castro, Michael J. Dee, Thomas R. Malek

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Much is known concerning the cellular and molecular basis for CD8 + T memory immune responses. Nevertheless, conditions that selectively support memory generation have remained elusive. In this study, we show that an immunization regimen that delivers TCR signals through a defined antigenic peptide, inflammatory signals through LPS, and growth and differentiation signals through the IL-2R initially favors Ag-specific CD8 + T cells to develop rapidly and substantially into T effector-memory cells by TCR transgenic OVA-specific OT-I CD8+ T cells. Amplified CD8+ T memory development depends upon a critical frequency of Ag-specific T cells and direct responsiveness to IL-2. A homologous prime-boost immunization protocol with transiently enhanced IL-2R signaling in normal mice led to persistent polyclonal Ag-specific CD8+ T cells that supported protective immunity to Listeria monocytogenes . These results identify a general approach for amplified T memory development that may be useful to optimize vaccines aimed at generating robust cell-mediated immunity.

Original languageEnglish (US)
Pages (from-to)4321-4330
Number of pages10
JournalJournal of Immunology
Volume189
Issue number9
DOIs
StatePublished - Nov 1 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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