Abstract
Chronic bilateral common carotid artery occlusion (BCCAO) induces moderate ischemia (oligemia) in the rat forebrain in the absence of overt neuronal damage. In situ hybridization for brain-derived neurotrophic factor (BDNF) mRNA was used to search for a molecular response to moderate ischemia. BDNF mRNA was significantly increased in the hippocampal granule cells at 6 h of occlusion (ANOVA, Tukey test P<0.05). At 1, 7 and 14 days BDNF mRNA levels returned to control levels. The frequency of BDNF gene expression at 6 h was 83%, which was significantly higher than the 7% incidence of histological injury in the hippocampus (Fisher's exact test, P<0.002). Cerebral blood flow was reduced to 75% of control levels in the hippocampus after 1 week of BCCAO when measured with the autoradiographic method. Measurements of tissue flow with a microprobe for laser Doppler flow excluded decreases into the ischemic range during the period when elevated gene expression was observed. Prolonged moderate ischemia (oligemia) is a sufficient stimulus for BDNF gene expression in the hippocampus. These molecular studies provide direct evidence for an involvement of the hippocampus in the BCCAO model.
| Original language | English |
|---|---|
| Pages (from-to) | 157-166 |
| Number of pages | 10 |
| Journal | Molecular Brain Research |
| Volume | 92 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Aug 15 2001 |
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Keywords
- Brain-derived neurotrophic factor
- Cerebral blood flow
- Hippocampus
- Oligemia
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
Cite this
Transient changes of brain-derived neurotrophic factor (BDNF) mRNA expression in hippocampus during moderate ischemia induced by chronic bilateral common carotid artery occlusions in the rat. / Schmidt-Kastner, Rainald; Truettner, Jessie; Lin, Baowan; Zhao, Weizhao; Saul, Isabel; Busto, Raul; Ginsberg, Myron.
In: Molecular Brain Research, Vol. 92, No. 1-2, 15.08.2001, p. 157-166.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transient changes of brain-derived neurotrophic factor (BDNF) mRNA expression in hippocampus during moderate ischemia induced by chronic bilateral common carotid artery occlusions in the rat
AU - Schmidt-Kastner, Rainald
AU - Truettner, Jessie
AU - Lin, Baowan
AU - Zhao, Weizhao
AU - Saul, Isabel
AU - Busto, Raul
AU - Ginsberg, Myron
PY - 2001/8/15
Y1 - 2001/8/15
N2 - Chronic bilateral common carotid artery occlusion (BCCAO) induces moderate ischemia (oligemia) in the rat forebrain in the absence of overt neuronal damage. In situ hybridization for brain-derived neurotrophic factor (BDNF) mRNA was used to search for a molecular response to moderate ischemia. BDNF mRNA was significantly increased in the hippocampal granule cells at 6 h of occlusion (ANOVA, Tukey test P<0.05). At 1, 7 and 14 days BDNF mRNA levels returned to control levels. The frequency of BDNF gene expression at 6 h was 83%, which was significantly higher than the 7% incidence of histological injury in the hippocampus (Fisher's exact test, P<0.002). Cerebral blood flow was reduced to 75% of control levels in the hippocampus after 1 week of BCCAO when measured with the autoradiographic method. Measurements of tissue flow with a microprobe for laser Doppler flow excluded decreases into the ischemic range during the period when elevated gene expression was observed. Prolonged moderate ischemia (oligemia) is a sufficient stimulus for BDNF gene expression in the hippocampus. These molecular studies provide direct evidence for an involvement of the hippocampus in the BCCAO model.
AB - Chronic bilateral common carotid artery occlusion (BCCAO) induces moderate ischemia (oligemia) in the rat forebrain in the absence of overt neuronal damage. In situ hybridization for brain-derived neurotrophic factor (BDNF) mRNA was used to search for a molecular response to moderate ischemia. BDNF mRNA was significantly increased in the hippocampal granule cells at 6 h of occlusion (ANOVA, Tukey test P<0.05). At 1, 7 and 14 days BDNF mRNA levels returned to control levels. The frequency of BDNF gene expression at 6 h was 83%, which was significantly higher than the 7% incidence of histological injury in the hippocampus (Fisher's exact test, P<0.002). Cerebral blood flow was reduced to 75% of control levels in the hippocampus after 1 week of BCCAO when measured with the autoradiographic method. Measurements of tissue flow with a microprobe for laser Doppler flow excluded decreases into the ischemic range during the period when elevated gene expression was observed. Prolonged moderate ischemia (oligemia) is a sufficient stimulus for BDNF gene expression in the hippocampus. These molecular studies provide direct evidence for an involvement of the hippocampus in the BCCAO model.
KW - Brain-derived neurotrophic factor
KW - Cerebral blood flow
KW - Hippocampus
KW - Oligemia
UR - http://www.scopus.com/inward/record.url?scp=0035882215&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035882215&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(01)00157-7
DO - 10.1016/S0169-328X(01)00157-7
M3 - Article
C2 - 11483252
AN - SCOPUS:0035882215
VL - 92
SP - 157
EP - 166
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -