Transient alteration of gene expression in adipose-derived stem cells using liposomal-driven protein extracts

Noelia Campillo, María I. Arribas, Nestor Vicente-Salar, Angela Catania, Miriam Ramírez-Domínguez, Juan A. Reig, Juan Domínguez-Bendala, Vicente Micol, Enrique Roche

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Recent works have proposed the use of proteins instead of DNA/RNA as molecular tools for the modulation of gene expression patterns in stem cells. To obtain insulin-producing cells, certain proteins including Pdx-1, Isl-1, Pax-6, and Neuro-D, can be expressed or modified with transduction domains in order to penetrate and reprogram target cells. However, numerous aspects must be taken in account, including the fact that not all proteins may be used in transduction protocols. Thus, new methods must be developed. In this report we studied whether liposomes can work as carriers to introduce reprogramming protein extracts inside adipose tissue mesenchymal stem cells. To this end, the cells were incubated for 8 h in the presence of liposomes containing MIN-6 protein extracts. Charged liposomes fused with 70% of the cells, transferring their contents and transiently (up to 48 h) changing the gene expression pattern for specific endocrine pancreatic genes coding for Insulin-I, Insulin-II, Glucagon, Isl-1, MafA, and Nkx-6.1. No expression of Foxa-2, Pax-6, and Pdx-1 was observed. Although preliminary, the obtained results suggest that the use of liposomes for reprogramming techniques opens new possibilities for cell therapy.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalCellular and Molecular Bioengineering
Volume7
Issue number1
DOIs
StatePublished - Mar 2014

Keywords

  • Adipose tissue
  • Adult stem cells
  • Cell reprogramming
  • Liposomes
  • Protein transduction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

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    Campillo, N., Arribas, M. I., Vicente-Salar, N., Catania, A., Ramírez-Domínguez, M., Reig, J. A., Domínguez-Bendala, J., Micol, V., & Roche, E. (2014). Transient alteration of gene expression in adipose-derived stem cells using liposomal-driven protein extracts. Cellular and Molecular Bioengineering, 7(1), 145-154. https://doi.org/10.1007/s12195-013-0298-3