Transgenic sugarcane plants resistant to stem borer attack

Ariel Arencibia, Roberto I. Vázquez, Dmitri Prieto, Pilar Téllez, Elva R. Carmona, Alberto Coego, Lázaro Hernández, Gustave A. De La Riva, Guillermo Selman-Housein

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

A truncated cryIA(b) gene encoding the active region of the Bacillus thuringiensis δ-endotoxin was expressed in transgenic sugarcane plants (Saccharum officinarum L.) under the control of the CaMV 35S promoter. Genetic transformation was accomplished by electroporation of intact cells. The levels of recombinant toxin were established and biological activity tests were performed against neonate sugarcane borer (Diatraea saccharalis F.) larvae. Transgenic sugarcane plants showed significant larvicidal activity despite the low expression of CryIA(b). Abbreviations: BT, Bacillus thuringiensis; BTK; Bacillus thuringiensis var. kurstaki; CaMV 35S, cauliflower mosaic virus promoter for the 35S polyprotein; ICP, insecticidal crystal protein; Tnos, nopaline synthetase gene terminator from Agrobacterium tumefaciens; SCSB, sugarcane stem borer (Diatraea saccharalis F.). tcryIA(B), truncated version of the BT cryIA(b) gene; TMV, tobacco mosaic virus.

Original languageEnglish (US)
Pages (from-to)247-255
Number of pages9
JournalMolecular Breeding
Volume3
Issue number4
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • δ-endotoxin
  • Bacillus thuringiensis
  • Diatraea
  • Sugarcane
  • Transgenic plant

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

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  • Cite this

    Arencibia, A., Vázquez, R. I., Prieto, D., Téllez, P., Carmona, E. R., Coego, A., Hernández, L., De La Riva, G. A., & Selman-Housein, G. (1997). Transgenic sugarcane plants resistant to stem borer attack. Molecular Breeding, 3(4), 247-255. https://doi.org/10.1023/A:1009616318854