Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects

Wayne Balkan, Gordon K. Klintworth, Cheryl B. Bock, Elwood Linney

Research output: Contribution to journalArticle

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Abstract

Retinoic acid receptors (RARs) modulate gene expression following association with retinoic acid (RA). In transient transfection, an RARα-β-galactosidase fusion protein (RAR-LacZ) was able to transactivate expression in the absence of RA. When expressed in the ocular lens of transgenic mice, this constitutively active RAR-LacZ fusion gene resulted in founder and progeny animals that exhibited cataracts and microphthalmia, both being characteristics of retinoid-induced teratogenesis. The transgenic phenotypes indicate that retinoid teratogenesis can be mimicked by expression of a constitutively active RAR-LacZ fusion protein in retinoid-sensitive tissues.

Original languageEnglish (US)
Pages (from-to)622-625
Number of pages4
JournalDevelopmental Biology
Volume151
Issue number2
DOIs
StatePublished - Jun 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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