Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects

Wayne E Balkan, Gordon K. Klintworth, Cheryl B. Bock, Elwood Linney

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Retinoic acid receptors (RARs) modulate gene expression following association with retinoic acid (RA). In transient transfection, an RARα-β-galactosidase fusion protein (RAR-LacZ) was able to transactivate expression in the absence of RA. When expressed in the ocular lens of transgenic mice, this constitutively active RAR-LacZ fusion gene resulted in founder and progeny animals that exhibited cataracts and microphthalmia, both being characteristics of retinoid-induced teratogenesis. The transgenic phenotypes indicate that retinoid teratogenesis can be mimicked by expression of a constitutively active RAR-LacZ fusion protein in retinoid-sensitive tissues.

Original languageEnglish
Pages (from-to)622-625
Number of pages4
JournalDevelopmental Biology
Volume151
Issue number2
StatePublished - Dec 1 1992
Externally publishedYes

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Retinoic Acid Receptors
Transgenic Mice
Lenses
Retinoids
Teratogenesis
Tretinoin
Galactosidases
Microphthalmos
Crystalline Lens
Lac Operon
Cataract
Transfection
Proteins
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Developmental Biology

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Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects. / Balkan, Wayne E; Klintworth, Gordon K.; Bock, Cheryl B.; Linney, Elwood.

In: Developmental Biology, Vol. 151, No. 2, 01.12.1992, p. 622-625.

Research output: Contribution to journalArticle

Balkan, Wayne E ; Klintworth, Gordon K. ; Bock, Cheryl B. ; Linney, Elwood. / Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects. In: Developmental Biology. 1992 ; Vol. 151, No. 2. pp. 622-625.
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