Transgenic indicator mice for studying activated retinoic acid receptors during development

Wayne E Balkan, Melissa Colbert, Cheryl Bock, Elwood Linney

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Retinoic acid (RA) receptors (RARs) are li-gand-inducible transcription factors that bind to specific DNA sequences associated with the regulatory regions of RAregulatable genes. Since RA has been implicated as an important factor both in normal development and in teratological studies, one would like to have a model system that detects the presence of activated receptors during development. We have constructed a recombinant reporter gene that has three copies of the RA response element (RARE) from the RARβ-2 proer 5′ to the herpes simplex virus thymidine kinase pro; this regulatory region is coupled to the bacterial β-gatosidase reporter gene. This construct was RA inducible in transient transfection assays in F9 embryonal carcinoma cells. Transgenic embryos with this reporter gene construct exhibited tricted and reproducible patterns of β-galactosidase activity during embryogenesis, beginning between gestational ages day 7.5 and 8.5. At day 8.5, β-galactosidase activity was detected in the closed neurotube and somites. Day 8.5 embryos, from pegnant females fed RA 14 hr earlier, exhibited a greater intensity and distribution of β-galactosidase activity. Similarly, at later stages of gestation, maternal RA exposure resulted in enhanced embryonic β-galactosidase expression. This type of transgenic indicator mouse should be useful in detailing the role of activated RARs during embryonic development.

Original languageEnglish
Pages (from-to)3347-3351
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number8
StatePublished - Dec 1 1992
Externally publishedYes

Fingerprint

Retinoic Acid Receptors
Galactosidases
Tretinoin
Transgenic Mice
Reporter Genes
Nucleic Acid Regulatory Sequences
Embryonic Development
Embryonic Structures
Embryonal Carcinoma Stem Cells
Somites
Thymidine Kinase
Response Elements
Simplexvirus
Gestational Age
Transfection
Transcription Factors
Mothers
Pregnancy
Genes

Keywords

  • Differentiation
  • Mouse embryos
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Transgenic indicator mice for studying activated retinoic acid receptors during development. / Balkan, Wayne E; Colbert, Melissa; Bock, Cheryl; Linney, Elwood.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 8, 01.12.1992, p. 3347-3351.

Research output: Contribution to journalArticle

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