Transgene-mediated expression of tumor necrosis factor soluble receptor attenuates morphine tolerance in rats

J. Sun, S. Liu, M. Mata, D. J. Fink, Shuanglin Hao

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Opiate/narcotic analgesics are the most effective treatments for chronic severe pain, but their clinical utility is often hampered by the development of analgesic tolerance. Recent evidence suggests chronic morphine may activate glial cells to release proinflammatory cytokines. In this study, we used herpes simplex virus (HSV) vector-based gene transfer to dorsal root ganglion to produce a local release of p55 tumor necrosis factor (TNF) soluble receptor in the spinal cord in rats with morphine tolerance. Subcutaneous inoculation of HSV vectors expressing p55 TNF soluble receptor into the plantar surface of the hindpaws enhanced the antinociceptive effect of acute morphine in rats. Subcutaneous inoculation of those vectors into hindpaws also delayed the development of chronic morphine tolerance in rats. TNF soluble receptor expressed by HSV vector reduced gene transcription of spinal TNFα and interleukin-1Β (IL-1Β) induced by repeated morphine. Furthermore, we found that TNF soluble receptor mediated by HSV reversed the upregulation of protein level of TNFα and IL-1Β and phosphorylation of p38 mitogen-activated protein kinase induced by repeated morphine. These results support the concept that proinflammatory cytokines may have an important role in the pathogenesis induced by morphine. This study provides a novel approach to treating morphine tolerance.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalGene Therapy
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Tumor Necrosis Factor Receptors
Transgenes
Morphine
Simplexvirus
Interleukin-1
Opiate Alkaloids
Tumor Necrosis Factor-alpha
Cytokines
Narcotics
Spinal Ganglia
p38 Mitogen-Activated Protein Kinases
Neuroglia
Chronic Pain
Genes
Analgesics
Spinal Cord
Up-Regulation
Phosphorylation

Keywords

  • cytokines
  • morphine tolerance
  • TNF soluble receptor

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Transgene-mediated expression of tumor necrosis factor soluble receptor attenuates morphine tolerance in rats. / Sun, J.; Liu, S.; Mata, M.; Fink, D. J.; Hao, Shuanglin.

In: Gene Therapy, Vol. 19, No. 1, 01.01.2012, p. 101-108.

Research output: Contribution to journalArticle

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