Transforming growth factor alpha treatment alters intracellular calcium levels in hair cells and protects them from ototoxic damage in vitro

Hinrich Staecker, Stefan Dazert, Brigitte Malgrange, Philippe P. Lefebvre, Allen F. Ryan, Thomas R. Van De Water

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

To determine if transforming growth factor alpha (TGFα) pretreatment protects hair cells from aminoglycoside induced injury by modifying their intracellular calcium concentration, we assayed hair cell calcium levels in organ of Corti explants both before and after aminoglycoside (i.e. neomycin, 10-3M) exposure either with or without growth factor pretreatment. After TGFα (500ng/ml) treatment, the intracellular calcium level of hair cells showed a five-fold increase as compared to the levels observed in the hair cells of control cultures. After ototoxin exposure, calcium levels in hair cells of control explants showed an increase relative to their baseline levels, while in the presence of growth factors pretreatment, hair cells showed a relative reduction in calcium levels. Pretreatment of organ of Corti explants afforded significant protection of hair cell stereocilia bundle morphology from ototoxic damage when compared to explants exposed to ototoxin alone. This study correlates a rise in hair cell calcium levels with the otoprotection of hair cells by TGFα in organ of Corti explants.

Original languageEnglish (US)
Pages (from-to)553-562
Number of pages10
JournalInternational Journal of Developmental Neuroscience
Volume15
Issue number4-5
DOIs
StatePublished - Jul 1 1997

Keywords

  • Aminoglycoside ototoxicity
  • Calcium levels
  • Growth factor protection
  • Hair cells
  • Organ of Corti explant

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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