Transforming growth factor-β receptor type I and type II expression during murine hair follicle development and cycling

R. Paus, K. Foitzik, P. Welker, S. Bulfone-Paus, S. Eichmuller

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

Although the TGF-β family of growth factors probably regulates skin and hair follicle development, its exact role is still quite ill-defined. Here, we characterize the correlative expression pattern of the interdependent high affinity receptor proteins for TGF-β1 and TGF-β3, TGF-β receptor type I (TGF-βRI) and TGF-β receptor type II (TGF-βRII), during hair follicle development and cycling in C57BL/6 mice. During neonatal follicle development, TGF-βRII immunoreactivity is confined to epithelial cells. Focal epidermal TGF-βRII expression is seen even before actual hair placode formation. In contrast to the TGF-βRII immunoreactivity in the outer root sheath, precortical hair matrix and inner root sheath cells were TGF-βRII negative during hair bulb morphogenesis. TGF-βRI (Alk-5) immunoreactivity largely overlapped the TGF-βRII expression pattern, but was more widespread. During hair follicle cycling in adolescent mice, TGF-βRII immunoreactivity was restricted to follicles, and was strikingly hair cycle dependent (maximal immunoreactivity; anagen VI and early catagen). Again, TGF-βRI (Alk-5) immunoreactivity co-localized with TGF-βRII immunoreactivity, but was more extensive. Reverse transcriptase polymerase chain reaction analysis of TGF- βRII mRNA confirmed peak transcript levels in back skin with most hair follicles in the anagen VI transformation. mRNA levels of TGF-βRI (Alk-5) did not vary significantly during the hair cycle, whereas those of TGF-βRI (threonine-serine kinase 7 L) declined during early anagen, and were maximal during the anagen-catagen transition. This provides a basis for defining the choreography of TGF-β-related signalling during hair follicle morphogenesis and cycling, introduces intraepidermal TGF-βRII immunoreactivity as a marker for imminent follicle development, and supports the concept that both TGF- βRII and TGF-βRI stimulation is involved in, but not restricted to, the control of catagen induction.

Original languageEnglish (US)
Pages (from-to)518-526
Number of pages9
JournalJournal of Investigative Dermatology
Volume109
Issue number4
DOIs
StatePublished - Jan 1 1997

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Keywords

  • Alk-5
  • Development
  • Hair growth
  • Tsk 7 L

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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