Transformation of rat bladder epithelial cells by introduction of a single oncogene

A. M. Mann, M. Stevenson, T. Masui, C. D. Borgeson, S. M. Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

Malignant transformation of cells in vitro requires the action of cooperating oncogenes. However, the action of a single activated oncogene, if placed under a strong constitutive promoter, is sufficient to transform in vitro established cells. We have investigated the role of cooperating oncogenes in the transformation of normal rat bladder epithelial cells. Effects of polyoma middle T, v-Ha-ras or activated rat c-Ha-ras, independently or in combination with v-myc on in vitro and in vivo behavior of rat bladder epithelial cells were studied. Introduction of polyoma middle T alone, c-Ha-ras or in some cases v-myc into 'normal' rat bladder epithelial cells was sufficient for full malignant transformation of these cells. In addition, introduction of polyoma middle T, activated c-Ha-ras or v-myc transformed cells into nude mice, resulted in development of highly invasive adenocarcinomas. These results indicate that full malignant transformation of normal rat bladder epithelial cells did not require the action of introduced cooperating oncogenes.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalOncogene Research
Volume6
Issue number1
StatePublished - 1991
Externally publishedYes

Keywords

  • Bladder cancer
  • In vitro transformation
  • Nude mice
  • Oncogenes

ASJC Scopus subject areas

  • Cancer Research

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