Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production

Fortunato Ciardiello, Nancy Hynes, Nancy Kim, Eva M. Valverius, Marc E. Lippman, David S. Salomon

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.

Original languageEnglish (US)
Pages (from-to)474-478
Number of pages5
JournalFEBS letters
Volume250
Issue number2
DOIs
StatePublished - Jul 3 1989

Keywords

  • Oncogene
  • Oncogene, neu
  • Oncogene, ras
  • Transformation
  • Transforming growth factor-α

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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