Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production

Fortunato Ciardiello; Nancy Hynes; Nancy Kim; Eva M. Valverius; Marc E. Lippman; David S. Salomon

doi:10.1016/0014-5793(89)80779-3

Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production

Fortunato Ciardiello, Nancy Hynes, Nancy Kim, Eva M. Valverius, Marc E. Lippman, David S. Salomon

Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21^ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.

Original language	English (US)
Pages (from-to)	474-478
Number of pages	5
Journal	FEBS letters
Volume	250
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)80779-3
State	Published - Jul 3 1989

Keywords

Oncogene
Oncogene, neu
Oncogene, ras
Transformation
Transforming growth factor-α

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production. / Ciardiello, Fortunato; Hynes, Nancy; Kim, Nancy; Valverius, Eva M.; Lippman, Marc E.; Salomon, David S.

In: FEBS letters, Vol. 250, No. 2, 03.07.1989, p. 474-478.

Research output: Contribution to journal › Article › peer-review

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title = "Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production",

abstract = "An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.",

keywords = "Oncogene, Oncogene, neu, Oncogene, ras, Transformation, Transforming growth factor-α",

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AU - Ciardiello, Fortunato

AU - Hynes, Nancy

AU - Kim, Nancy

AU - Valverius, Eva M.

AU - Lippman, Marc E.

AU - Salomon, David S.

PY - 1989/7/3

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N2 - An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.

AB - An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.

KW - Oncogene

KW - Oncogene, neu

KW - Oncogene, ras

KW - Transformation

KW - Transforming growth factor-α

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Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production

Abstract

Keywords

ASJC Scopus subject areas

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