Abstract
An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.
| Original language | English |
|---|---|
| Pages (from-to) | 474-478 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 250 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 3 1989 |
| Externally published | Yes |
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Keywords
- Oncogene
- Oncogene, neu
- Oncogene, ras
- Transformation
- Transforming growth factor-α
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production. / Ciardiello, Fortunato; Hynes, Nancy; Kim, Nancy; Valverius, Eva M.; Lippman, Marc E; Salomon, David S.
In: FEBS Letters, Vol. 250, No. 2, 03.07.1989, p. 474-478.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transformation of mouse mammary epithelial cells with the Ha-ras but not with the neu oncogene results in a gene dosage-dependent increase in transforming growth factor-α production
AU - Ciardiello, Fortunato
AU - Hynes, Nancy
AU - Kim, Nancy
AU - Valverius, Eva M.
AU - Lippman, Marc E
AU - Salomon, David S.
PY - 1989/7/3
Y1 - 1989/7/3
N2 - An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.
AB - An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.
KW - Oncogene
KW - Oncogene, neu
KW - Oncogene, ras
KW - Transformation
KW - Transforming growth factor-α
UR - http://www.scopus.com/inward/record.url?scp=0024339329&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024339329&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(89)80779-3
DO - 10.1016/0014-5793(89)80779-3
M3 - Article
C2 - 2568949
AN - SCOPUS:0024339329
VL - 250
SP - 474
EP - 478
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -