Abstract
An enhanced expression of transforming growth factor-α (TGFα) was demonstrated in two clones of NOG-8 mouse mammary epithelial cells, NOG-8 SR1 and NOG-8 SR2, that have been transformed by a v-Ha-ras oncogene. The amount of TGFα production in NOG-8 SR1 and NOG-8 SR2 cells was dependent on the level of p21ras expression in these clones, which directly correlated with their cloning efficiency in soft agar. There was also a decrease in the number of epidermal growth factor (EGF) receptors on the NOG-8 SR1 and NOG-8 SR2 cells that is proportional to the amount of TGFα secreted. These effects were specific for ras because neu-transformed NOG-8 cells grew in soft agar at a comparable level to NOG-8 SR2 cells yet did not show any increase in TGFα production or change in EGF receptor expression.
Original language | English (US) |
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Pages (from-to) | 474-478 |
Number of pages | 5 |
Journal | FEBS letters |
Volume | 250 |
Issue number | 2 |
DOIs | |
State | Published - Jul 3 1989 |
Keywords
- Oncogene
- Oncogene, neu
- Oncogene, ras
- Transformation
- Transforming growth factor-α
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology