Transfer RNA is a substrate for RNase D in vivo

J. Zhang, Murray P Deutscher

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

RNase D is a 3'-exonuclease whose in vitro specificity has suggested a role in tRNA processing. However, since mutant Escherichia coli strains devoid of RNase D display a normal phenotype, it has not been possible to ascertain the enzyme's fucntion or even to determine which RNA is its substrate in vivo. Here we show that transformation of strains devoid of tRNA nucleotidyl-transferase with a multicopy plasmid carrying the rnd+ gene leads to extremely slow growth due to elevated levels of RNase D activity. Analysis of such a slow-growing strain revealed that less tRNA is present in the cell and that the tRNA that could be recovered is substantially damaged. These studies demonstrate that RNase D can act at the 3' terminus of tRNA in vivo, and they support the conclusion that RNase D participates in tRNA metabolism.

Original languageEnglish
Pages (from-to)17909-17912
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number34
StatePublished - Dec 1 1988
Externally publishedYes

Fingerprint

Ribonuclease III
Transfer RNA
Substrates
spleen exonuclease
Transferases
Metabolism
Escherichia coli
Plasmids
Genes
RNA
Phenotype
Enzymes
Growth
Processing

ASJC Scopus subject areas

  • Biochemistry

Cite this

Zhang, J., & Deutscher, M. P. (1988). Transfer RNA is a substrate for RNase D in vivo. Journal of Biological Chemistry, 263(34), 17909-17912.

Transfer RNA is a substrate for RNase D in vivo. / Zhang, J.; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 263, No. 34, 01.12.1988, p. 17909-17912.

Research output: Contribution to journalArticle

Zhang, J & Deutscher, MP 1988, 'Transfer RNA is a substrate for RNase D in vivo', Journal of Biological Chemistry, vol. 263, no. 34, pp. 17909-17912.
Zhang J, Deutscher MP. Transfer RNA is a substrate for RNase D in vivo. Journal of Biological Chemistry. 1988 Dec 1;263(34):17909-17912.
Zhang, J. ; Deutscher, Murray P. / Transfer RNA is a substrate for RNase D in vivo. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 34. pp. 17909-17912.
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