Transfer RNA analysis during the reproductive cycle of a freshwater teleost, H. fossilis

Malancha Sarkar, Subbayan Pochi, Manjula Vinayak

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Transfer RNA was analyzed qualitatively as well as quantitatively from ovaries of the fresh water teleost Heteropneustes fossilis for twelve months. The tRNA samples were found to be pure and devoid of any high molecular weight RNA or DNA contaminations. The quantity of tRNA as well as its biological activity, assayed by in vitro aminoacylation using homologous aminoacyl tRNA synthetases, were found to be higher during resting and preparatory (pre-vitellogenic) phases, i.e. from November to March, as compared to vitellogenic and spawning phases of the fish, i.e. from April to October. The highest tRNA pool and its activity was found in the month of February, which coincides with the early preparatory phase. The results indicate that the accumulation of active tRNA starts in the resting phase. Such an accumulation of tRNA may be a part of the enrichment of mature eggs with complete translational machinery before ovulation in order to cope with the high rate of protein synthesis after fertilization.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalMolecular Biology Reports
Volume20
Issue number1
DOIs
StatePublished - Jul 1 1994
Externally publishedYes

Fingerprint

Transfer RNA
Fresh Water
DNA Contamination
Aminoacylation
Amino Acyl-tRNA Synthetases
Catfishes
Ovulation
Bioactivity
Fertilization
Fish
Eggs
Machinery
Ovary
Fishes
Contamination
Molecular Weight
Molecular weight
RNA
Water
DNA

Keywords

  • aaRS
  • aminoacylation
  • reproductive cycle
  • tRNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

Transfer RNA analysis during the reproductive cycle of a freshwater teleost, H. fossilis. / Sarkar, Malancha; Pochi, Subbayan; Vinayak, Manjula.

In: Molecular Biology Reports, Vol. 20, No. 1, 01.07.1994, p. 9-13.

Research output: Contribution to journalArticle

Sarkar, Malancha ; Pochi, Subbayan ; Vinayak, Manjula. / Transfer RNA analysis during the reproductive cycle of a freshwater teleost, H. fossilis. In: Molecular Biology Reports. 1994 ; Vol. 20, No. 1. pp. 9-13.
@article{0a6af19fed954a3fa2f5b75cd9f14728,
title = "Transfer RNA analysis during the reproductive cycle of a freshwater teleost, H. fossilis",
abstract = "Transfer RNA was analyzed qualitatively as well as quantitatively from ovaries of the fresh water teleost Heteropneustes fossilis for twelve months. The tRNA samples were found to be pure and devoid of any high molecular weight RNA or DNA contaminations. The quantity of tRNA as well as its biological activity, assayed by in vitro aminoacylation using homologous aminoacyl tRNA synthetases, were found to be higher during resting and preparatory (pre-vitellogenic) phases, i.e. from November to March, as compared to vitellogenic and spawning phases of the fish, i.e. from April to October. The highest tRNA pool and its activity was found in the month of February, which coincides with the early preparatory phase. The results indicate that the accumulation of active tRNA starts in the resting phase. Such an accumulation of tRNA may be a part of the enrichment of mature eggs with complete translational machinery before ovulation in order to cope with the high rate of protein synthesis after fertilization.",
keywords = "aaRS, aminoacylation, reproductive cycle, tRNA",
author = "Malancha Sarkar and Subbayan Pochi and Manjula Vinayak",
year = "1994",
month = "7",
day = "1",
doi = "10.1007/BF00999849",
language = "English",
volume = "20",
pages = "9--13",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Transfer RNA analysis during the reproductive cycle of a freshwater teleost, H. fossilis

AU - Sarkar, Malancha

AU - Pochi, Subbayan

AU - Vinayak, Manjula

PY - 1994/7/1

Y1 - 1994/7/1

N2 - Transfer RNA was analyzed qualitatively as well as quantitatively from ovaries of the fresh water teleost Heteropneustes fossilis for twelve months. The tRNA samples were found to be pure and devoid of any high molecular weight RNA or DNA contaminations. The quantity of tRNA as well as its biological activity, assayed by in vitro aminoacylation using homologous aminoacyl tRNA synthetases, were found to be higher during resting and preparatory (pre-vitellogenic) phases, i.e. from November to March, as compared to vitellogenic and spawning phases of the fish, i.e. from April to October. The highest tRNA pool and its activity was found in the month of February, which coincides with the early preparatory phase. The results indicate that the accumulation of active tRNA starts in the resting phase. Such an accumulation of tRNA may be a part of the enrichment of mature eggs with complete translational machinery before ovulation in order to cope with the high rate of protein synthesis after fertilization.

AB - Transfer RNA was analyzed qualitatively as well as quantitatively from ovaries of the fresh water teleost Heteropneustes fossilis for twelve months. The tRNA samples were found to be pure and devoid of any high molecular weight RNA or DNA contaminations. The quantity of tRNA as well as its biological activity, assayed by in vitro aminoacylation using homologous aminoacyl tRNA synthetases, were found to be higher during resting and preparatory (pre-vitellogenic) phases, i.e. from November to March, as compared to vitellogenic and spawning phases of the fish, i.e. from April to October. The highest tRNA pool and its activity was found in the month of February, which coincides with the early preparatory phase. The results indicate that the accumulation of active tRNA starts in the resting phase. Such an accumulation of tRNA may be a part of the enrichment of mature eggs with complete translational machinery before ovulation in order to cope with the high rate of protein synthesis after fertilization.

KW - aaRS

KW - aminoacylation

KW - reproductive cycle

KW - tRNA

UR - http://www.scopus.com/inward/record.url?scp=0028467943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028467943&partnerID=8YFLogxK

U2 - 10.1007/BF00999849

DO - 10.1007/BF00999849

M3 - Article

VL - 20

SP - 9

EP - 13

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 1

ER -