Abstract
It was previously shown that 10-6 M dexamethasone phosphate induces an increase in phenylalanine acceptor activity in hepatoma tissue culture cells in vivo after a characteristic lag of about 1 hr. The authors now present data demonstrating the increase as phenylalanyl transfer RNA in an in vitro aminoacylation system. No significant alterations in phenylalanyl aminoacyl transferase were found. A general increase in other species of transfer RNA was not detected. Separation of phenylalanyl tRNA into two isoaccepting species by reverse phase chromatography revealed that the increase in phenylalanyl tRNA was restricted to only one of the two phenylalanine chargeable transfer RNAs.
| Original language | English |
|---|---|
| Pages (from-to) | 262-266 |
| Number of pages | 5 |
| Journal | Endocrinology |
| Volume | 94 |
| Issue number | 1 |
| State | Published - Jan 1 1974 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Transfer ribonucleic acid in hepatoma tissue culture cells. II. Dexamethasone phosphate induction of tRNA(phe) analyzed under cell free conditions. / Lippman, Marc E; Yang, S. S.; Thompson, E. B.
In: Endocrinology, Vol. 94, No. 1, 01.01.1974, p. 262-266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transfer ribonucleic acid in hepatoma tissue culture cells. II. Dexamethasone phosphate induction of tRNA(phe) analyzed under cell free conditions
AU - Lippman, Marc E
AU - Yang, S. S.
AU - Thompson, E. B.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - It was previously shown that 10-6 M dexamethasone phosphate induces an increase in phenylalanine acceptor activity in hepatoma tissue culture cells in vivo after a characteristic lag of about 1 hr. The authors now present data demonstrating the increase as phenylalanyl transfer RNA in an in vitro aminoacylation system. No significant alterations in phenylalanyl aminoacyl transferase were found. A general increase in other species of transfer RNA was not detected. Separation of phenylalanyl tRNA into two isoaccepting species by reverse phase chromatography revealed that the increase in phenylalanyl tRNA was restricted to only one of the two phenylalanine chargeable transfer RNAs.
AB - It was previously shown that 10-6 M dexamethasone phosphate induces an increase in phenylalanine acceptor activity in hepatoma tissue culture cells in vivo after a characteristic lag of about 1 hr. The authors now present data demonstrating the increase as phenylalanyl transfer RNA in an in vitro aminoacylation system. No significant alterations in phenylalanyl aminoacyl transferase were found. A general increase in other species of transfer RNA was not detected. Separation of phenylalanyl tRNA into two isoaccepting species by reverse phase chromatography revealed that the increase in phenylalanyl tRNA was restricted to only one of the two phenylalanine chargeable transfer RNAs.
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UR - http://www.scopus.com/inward/citedby.url?scp=0015987149&partnerID=8YFLogxK
M3 - Article
C2 - 4358031
AN - SCOPUS:0015987149
VL - 94
SP - 262
EP - 266
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 1
ER -