Transfer ribonucleic acid in hepatoma tissue culture cells. II. Dexamethasone phosphate induction of tRNA(phe) analyzed under cell free conditions

Marc E Lippman, S. S. Yang, E. B. Thompson

Research output: Contribution to journalArticle

Abstract

It was previously shown that 10-6 M dexamethasone phosphate induces an increase in phenylalanine acceptor activity in hepatoma tissue culture cells in vivo after a characteristic lag of about 1 hr. The authors now present data demonstrating the increase as phenylalanyl transfer RNA in an in vitro aminoacylation system. No significant alterations in phenylalanyl aminoacyl transferase were found. A general increase in other species of transfer RNA was not detected. Separation of phenylalanyl tRNA into two isoaccepting species by reverse phase chromatography revealed that the increase in phenylalanyl tRNA was restricted to only one of the two phenylalanine chargeable transfer RNAs.

Original languageEnglish
Pages (from-to)262-266
Number of pages5
JournalEndocrinology
Volume94
Issue number1
StatePublished - Jan 1 1974
Externally publishedYes

Fingerprint

dexamethasone 21-phosphate
RNA, Transfer, Phe
Transfer RNA
Hepatocellular Carcinoma
Cell Culture Techniques
Phenylalanine
Aminoacylation
Reverse-Phase Chromatography
Transferases

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Transfer ribonucleic acid in hepatoma tissue culture cells. II. Dexamethasone phosphate induction of tRNA(phe) analyzed under cell free conditions. / Lippman, Marc E; Yang, S. S.; Thompson, E. B.

In: Endocrinology, Vol. 94, No. 1, 01.01.1974, p. 262-266.

Research output: Contribution to journalArticle

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