Transfection of molecular beacons in microchannels under flow and no-flow conditions

Na Li, P. K. Wong, J. Lin, C. M. Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

In this work, transfection of molecular beacons in traditional cell culture dishes and microfluidic cell culture systems with or without flows were comparatively studied by both computational and experimental methods. The comparison between static microfluidic systems and cell culture dishes reveals that the transfection of molecular beacons is controlled by both diffusion and reaction. The study on the microfluidic system with a range of fluid shear stresses and mass transfer rates implies that under high levels of shear stress the disruption of the binding between transfection reagent/molecular beacon complexes and cell membrane is dominant over the enhanced mass transfer.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages260-263
Number of pages4
DOIs
StatePublished - Aug 29 2008
Externally publishedYes
Event21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States
Duration: Jan 13 2008Jan 17 2008

Other

Other21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
CountryUnited States
CityTucson, AZ
Period1/13/081/17/08

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ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering
  • Control and Systems Engineering

Cite this

Li, N., Wong, P. K., Lin, J., & Ho, C. M. (2008). Transfection of molecular beacons in microchannels under flow and no-flow conditions. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 260-263). [4443642] https://doi.org/10.1109/MEMSYS.2008.4443642