Transfection of lymphoblastoid cells using DNA-loaded reconstituted Sendai virus envelopes: Expression of transfected DNA and selection of transfected cells

I. M. Shapiro, Mario Stevenson, Faruk Sinangil, David J. Volsky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The American Burkitt's lymphoma cell line Loukes was cotransfected with cloned BamHI K fragment of EBV DNA and a vector pSV2neo. Reconstituted Sendai virus envelopes (RSVE) loaded with DNA were used for efficient gene transfer. Two cell lines have been obtained following culture in the presence of geneticin sulfate (G-418). Messenger RNA from both transfected DNAs was expressed during the whole period of observation, 42 days after transfection. This method provides a relatively simple and efficient means for selection of lymphoblastoid cells expressing a transfected gene.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalSomatic Cell and Molecular Genetics
Volume12
Issue number4
DOIs
StatePublished - Jul 1 1986
Externally publishedYes

Fingerprint

Sendai virus
Transfection
DNA
Cell Line
Burkitt Lymphoma
Human Herpesvirus 4
Genes
Observation
Messenger RNA
antibiotic G 418

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Transfection of lymphoblastoid cells using DNA-loaded reconstituted Sendai virus envelopes : Expression of transfected DNA and selection of transfected cells. / Shapiro, I. M.; Stevenson, Mario; Sinangil, Faruk; Volsky, David J.

In: Somatic Cell and Molecular Genetics, Vol. 12, No. 4, 01.07.1986, p. 351-356.

Research output: Contribution to journalArticle

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