Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity

P. O. Berggren, A. Hallberg, N. Welsh, P. Arkhammar, T. Nilsson, M. Welsh

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Abstract

Pancreatic islet β-cells and insulin-producing RINm5F cells were electroporated in the presence of the c-Ha-ras oncogene, to assess the possible involvement of the encoded product in coupling extracellular receptors to phospholipase C. After two days the c-Ha-ras-transfected cells increased their expression of c-Ha-ras mRNA. These cells were also found to contain more [3H]InsP3, suggesting an increased basal (non-ligand-activated) phospholipase C activity. In addition, the transfected cells were unable to respond to ligand (bombesin) activation of phospholipase C. The ras-transfected insulin-producing cells showed enhanced phosphorylation of a 200 kDa substrate crossreacting with an antibody to an 80 kDa protein kinase C substrate. The phorbol ester 12-O-tetradecanoyl 13-acetate and bombesin also induced phosphorylation of the 200 kDa substrate. All of these changes occurred without changes in the rates of [3H]thymidine incorporation. The results suggest that the mutated c-Ha-ras oncogene directly or indirectly stimulates the basal phospholipase C activity of these cells.

Original languageEnglish (US)
Pages (from-to)701-707
Number of pages7
JournalBiochemical Journal
Volume259
Issue number3
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Berggren, P. O., Hallberg, A., Welsh, N., Arkhammar, P., Nilsson, T., & Welsh, M. (1989). Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity. Biochemical Journal, 259(3), 701-707. https://doi.org/10.1042/bj2590701