Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity

P. O. Berggren, A. Hallberg, N. Welsh, P. Arkhammar, T. Nilsson, M. Welsh

Research output: Contribution to journalArticle

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Abstract

Pancreatic islet β-cells and insulin-producing RINm5F cells were electroporated in the presence of the c-Ha-ras oncogene, to assess the possible involvement of the encoded product in coupling extracellular receptors to phospholipase C. After two days the c-Ha-ras-transfected cells increased their expression of c-Ha-ras mRNA. These cells were also found to contain more [3H]InsP3, suggesting an increased basal (non-ligand-activated) phospholipase C activity. In addition, the transfected cells were unable to respond to ligand (bombesin) activation of phospholipase C. The ras-transfected insulin-producing cells showed enhanced phosphorylation of a 200 kDa substrate crossreacting with an antibody to an 80 kDa protein kinase C substrate. The phorbol ester 12-O-tetradecanoyl 13-acetate and bombesin also induced phosphorylation of the 200 kDa substrate. All of these changes occurred without changes in the rates of [3H]thymidine incorporation. The results suggest that the mutated c-Ha-ras oncogene directly or indirectly stimulates the basal phospholipase C activity of these cells.

Original languageEnglish
Pages (from-to)701-707
Number of pages7
JournalBiochemical Journal
Volume259
Issue number3
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

ras Genes
Type C Phospholipases
Transfection
Insulin
Bombesin
Phosphorylation
Substrates
Islets of Langerhans
Phorbol Esters
Thymidine
Protein Kinase C
Acetates
Chemical activation
Cells
Ligands
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Berggren, P. O., Hallberg, A., Welsh, N., Arkhammar, P., Nilsson, T., & Welsh, M. (1989). Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity. Biochemical Journal, 259(3), 701-707.

Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity. / Berggren, P. O.; Hallberg, A.; Welsh, N.; Arkhammar, P.; Nilsson, T.; Welsh, M.

In: Biochemical Journal, Vol. 259, No. 3, 01.01.1989, p. 701-707.

Research output: Contribution to journalArticle

Berggren, PO, Hallberg, A, Welsh, N, Arkhammar, P, Nilsson, T & Welsh, M 1989, 'Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity', Biochemical Journal, vol. 259, no. 3, pp. 701-707.
Berggren PO, Hallberg A, Welsh N, Arkhammar P, Nilsson T, Welsh M. Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity. Biochemical Journal. 1989 Jan 1;259(3):701-707.
Berggren, P. O. ; Hallberg, A. ; Welsh, N. ; Arkhammar, P. ; Nilsson, T. ; Welsh, M. / Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity. In: Biochemical Journal. 1989 ; Vol. 259, No. 3. pp. 701-707.
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