Transcriptomic Analyses of Inner Ear Sensory Epithelia in Zebrafish

Qi Yao, Lingyu Wang, Rahul Mittal, Denise Yan, Michael T. Richmond, Steven Denyer, Teresa Requena, Kaili Liu, Gaurav K. Varshney, Zhongmin Lu, Xue Zhong Liu

Research output: Contribution to journalArticle

Abstract

Analysis of gene expression has the potential to assist in the understanding of multiple cellular processes including proliferation, cell-fate specification, senesence, and activity in both healthy and disease states. Zebrafish model has been increasingly used to understand the process of hearing and the development of the vertebrate auditory system. Within the zebrafish inner ear, there are three otolith organs, each containing a sensory macula of hair cells. The saccular macula is primarily involved in hearing, the utricular macula is primarily involved in balance and the function of the lagenar macula is not completely understood. The goal of this study is to understand the transcriptional differences in the sensory macula associated with different otolith organs with the intention of understanding the genetic mechanisms responsible for the distinct role each organ plays in sensory perception. The sensory maculae of the saccule, utricle, and lagena were dissected out of adult Et(krt4:GFP)sqet4 zebrafish expressing green fluorescent protein in hair cells for transcriptional analysis. The total RNAs of the maculae were isolated and analyzed by RNA GeneChip microarray. Several of the differentially expressed genes are known to be involved in deafness, otolith development and balance. Gene expression among these otolith organs was very well conserved with less than 10% of genes showing differential expression. Data from this study will help to elucidate which genes are involved in hearing and balance. Furthermore, the findings of this study will assist in the development of the zebrafish model for human hearing and balance disorders. Anat Rec, 303:527–543, 2020.

Original languageEnglish (US)
Pages (from-to)527-543
Number of pages17
JournalAnatomical Record
Volume303
Issue number3
DOIs
StatePublished - Mar 1 2020

Keywords

  • hearing
  • microarray
  • neurosensory epithelium
  • transcriptome analysis
  • zebrafish inner ear

ASJC Scopus subject areas

  • Anatomy
  • Biotechnology
  • Histology
  • Ecology, Evolution, Behavior and Systematics

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  • Cite this

    Yao, Q., Wang, L., Mittal, R., Yan, D., Richmond, M. T., Denyer, S., Requena, T., Liu, K., Varshney, G. K., Lu, Z., & Liu, X. Z. (2020). Transcriptomic Analyses of Inner Ear Sensory Epithelia in Zebrafish. Anatomical Record, 303(3), 527-543. https://doi.org/10.1002/ar.24331