Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization

Courtney A. Deck, Sheldon J. McKay, Tristan J. Fiedler, Christophe M R Lemoine, Makiko Kajimura, C. Michele Nawata, Chris M. Wood, Patrick J. Walsh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Prior studies of the elasmobranch rectal gland have demonstrated that feeding induces profound and rapid up regulation of the gland's ability to secrete concentrated NaCl solutions and the metabolic capacity to support this highly ATP consuming process. We undertook the current study to attempt to determine the degree to which up regulation of mRNA transcription was involved in the gland's activation. cDNA libraries were created from mRNA isolated from rectal glands of fasted (7 days post-feeding) and fed (6 h and 22 h post-feeding) spiny dogfish sharks (Squalus acanthias), and the libraries were subjected to suppression subtractive hybridization (SSH) analysis. Quantitative real time PCR (qPCR) was also used to ascertain the mRNA expression of several genes revealed by the SSH analysis. In total the treatments changed the abundance of 170 transcripts, with 103 up regulated by feeding, and 67 up regulated by fasting. While many of the changes took place in 'expected' Gene Ontology (GO) categories (e.g., metabolism, transport, structural proteins, DNA and RNA turnover, etc.), KEGG analysis revealed a number of categories which identify oxidative stress as a topic of interest for the gland. GO analysis also revealed that branched chain essential amino acids (e.g., valine, leucine, isoleucine) are potential metabolic fuels for the rectal gland. In addition, up regulation of transcripts for many genes in the anticipated GO categories did not agree (i.e., fasting down regulated in feeding treatments) with previously observed increases in their respective proteins/enzyme activities. These results suggest an 'anticipatory' storage of selected mRNAs which presumably supports the rapid translation of proteins upon feeding activation of the gland.

Original languageEnglish
Pages (from-to)334-343
Number of pages10
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume8
Issue number4
DOIs
StatePublished - Oct 31 2013

Fingerprint

Squalus acanthias
Salt Gland
Sharks
Transcriptome
Gene Ontology
Genes
Messenger RNA
Up-Regulation
Ontology
Fasting
Elasmobranchii
Branched Chain Amino Acids
Aptitude
Essential Amino Acids
Isoleucine
Valine
Protein Biosynthesis
Chemical activation
Gene Library
Leucine

Keywords

  • Elasmobranchs
  • Feeding
  • Ion transport
  • Metabolism
  • Rectal gland
  • Transcript regulation

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology
  • Physiology

Cite this

Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization. / Deck, Courtney A.; McKay, Sheldon J.; Fiedler, Tristan J.; Lemoine, Christophe M R; Kajimura, Makiko; Nawata, C. Michele; Wood, Chris M.; Walsh, Patrick J.

In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 8, No. 4, 31.10.2013, p. 334-343.

Research output: Contribution to journalArticle

Deck, Courtney A. ; McKay, Sheldon J. ; Fiedler, Tristan J. ; Lemoine, Christophe M R ; Kajimura, Makiko ; Nawata, C. Michele ; Wood, Chris M. ; Walsh, Patrick J. / Transcriptome responses in the rectal gland of fed and fasted spiny dogfish shark (Squalus acanthias) determined by suppression subtractive hybridization. In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2013 ; Vol. 8, No. 4. pp. 334-343.
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AU - Fiedler, Tristan J.

AU - Lemoine, Christophe M R

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AU - Wood, Chris M.

AU - Walsh, Patrick J.

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