Transcriptional regulation by the estrogen receptor of antioxidative stress enzymes and its functional implications

Monica M. Montano, Huayun Deng, Min Liu, Xiaoyan Sun, Rakesh Singal

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


We previously reported that antiestrogen-liganded estrogen receptor β (ERβ) transcriptionally activates the major detoxifying enzyme quinone reductase (QR) (NAD(P)H:quinone oxidoreductase). Our studies also indicate that upregulation of QR, either by overexpression or induction by tamoxifen, can protect breast cells against oxidative DNA damage caused by estrogen metabolites. We now report on the upregulation of glutathione S-transferases Pi (GST-Pi) and gamma-glutamylcysteine synthetase heavy subunit (GCSh) expression by antiestrogens. Studies indicate the regulation of GST-Pi and GCSh transcriptional activity by ER. While ER regulation is mediated by an electrophile response element (EpRE), we identified mechanistic differences in the involvement of other transcription factors. Regardless of these differences, ERβ-mediated regulation of GST-Pi and GCSh point towards an important role for ERβ in cellular protection against oxidative stress. A protective role is supported by our observation of inhibition of estrogen-induced DNA damage upon upregulation of GST-Pi and GCSh expression.

Original languageEnglish (US)
Pages (from-to)2442-2453
Number of pages12
Issue number14
StatePublished - Apr 1 2004


  • Antiestrogens
  • Estrogen receptor
  • Gamma-glutamylcysteine synthetase
  • Glutathione-S-transferase

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics


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