Transcriptional profiling of intrinsic PNS factors in the postnatal mouse

Robin P. Smith, Jessica K. Lerch-Haner, Jose R. Pardinas, William J. Buchser, John L. Bixby, Vance P. Lemmon

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Neurons in the peripheral nervous system (PNS) display a higher capacity to regenerate after injury than those in the central nervous system, suggesting cell specific transcriptional modules underlying axon growth and inhibition. We report a systems biology based search for PNS specific transcription factors (TFs). Messenger RNAs enriched in dorsal root ganglion (DRG) neurons compared to cerebellar granule neurons (CGNs) were identified using subtractive hybridization and DNA microarray approaches. Network and transcription factor binding site enrichment analyses were used to further identify TFs that may be differentially active. Combining these techniques, we identified 32 TFs likely to be enriched and/or active in the PNS. Twenty-five of these TFs were then tested for an ability to promote CNS neurite outgrowth in an overexpression screen. Real-time PCR and immunohistochemical studies confirmed that one representative TF, STAT3, is intrinsic to PNS neurons, and that constitutively active STAT3 is sufficient to promote CGN neurite outgrowth.

Original languageEnglish (US)
Pages (from-to)32-44
Number of pages13
JournalMolecular and Cellular Neuroscience
Volume46
Issue number1
DOIs
StatePublished - Jan 1 2011

Keywords

  • Cerebellar granule neuron
  • Dorsal root ganglion
  • High content analysis
  • Screen
  • STAT3
  • Systems biology
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'Transcriptional profiling of intrinsic PNS factors in the postnatal mouse'. Together they form a unique fingerprint.

  • Cite this