Transcriptional Profile of HIV-induced Nuclear Translocation of Amyloid β in Brain Endothelial Cells

Ibolya E. András, Evadnie Rampersaud, Sung Yong Eum, Michal Toborek

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background and Aims: Increased amyloid deposition in HIV-infected brains may contribute to the pathogenesis of neurocognitive dysfunction in infected patients. We have previously shown that exposure to HIV results in enhanced amyloid β (Aβ) levels in human brain microvascular endothelial cells, suggesting that brain endothelial cells contribute to accumulation of Aβ in HIV-infected brains. Importantly, Aβ not only accumulates in the cytoplasm of HIV-exposed cells but also enters the nuclei of brain endothelial cells. Methods: cDNA microarray analysis was performed in order to examine changes in the transcriptional profile associated with Aβ nuclear entry in the presence of HIV-1. Results: Gene network analysis indicated that inhibition of nuclear entry of Aβ resulted in enrichment in gene sets involved in apoptosis and survival, endoplasmic reticulum stress response, immune response, cell cycle, DNA damage, oxidative stress, cytoskeleton remodeling and transforming growth factor β (TGFβ) receptor signaling. Conclusions: The obtained data indicate that HIV-induced Aβ nuclear uptake affects several cellular stress-related pathways relevant for HIV-induced Aβ pathology.

Original languageEnglish (US)
Pages (from-to)744-752
Number of pages9
JournalArchives of Medical Research
Volume45
Issue number8
DOIs
StatePublished - Nov 1 2014

Keywords

  • Amyloid beta
  • Blood-brain barrier
  • DNA microarray
  • HIV

ASJC Scopus subject areas

  • Medicine(all)

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