Abstract
The strands of the four bacteriophage N4 DNA fragments derived by HhaI endonuclease digestion have been separated on agarose gels, and the eight fragments obtained have been related to the two complementary strands of N4 DNA. We have used the HpaI restriction endonuclease fragments (Zivin et al., 1980) and single-stranded fragments of HhaI-digested N4 DNA as probes to determine the temporal appearance, localization and polarity of N4 transcripts. These experiments have allowed us to localize on the N4 genome the two previously characterized classes (early and middle RNAs) of N4 rifampicin-resistant RNAs (Vander Laan et al., 1977) and to detect a new class of N4 RNAs, which are synthesized late in infection. The synthesis of late N4 RNAs is rifampicin sensitive and, as we show in this paper, requires the activity of the Escherichia coli RNA polymerase.
| Original language | English |
|---|---|
| Pages (from-to) | 335-356 |
| Number of pages | 22 |
| Journal | Journal of Molecular Biology |
| Volume | 152 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 25 1981 |
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ASJC Scopus subject areas
- Virology
Cite this
Transcriptional map of bacteriophage N4. Location and polarity of N4 RNAs. / Zivin, R.; Zehring, W.; Rothman-Denes, L. B.
In: Journal of Molecular Biology, Vol. 152, No. 2, 25.10.1981, p. 335-356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcriptional map of bacteriophage N4. Location and polarity of N4 RNAs
AU - Zivin, R.
AU - Zehring, W.
AU - Rothman-Denes, L. B.
PY - 1981/10/25
Y1 - 1981/10/25
N2 - The strands of the four bacteriophage N4 DNA fragments derived by HhaI endonuclease digestion have been separated on agarose gels, and the eight fragments obtained have been related to the two complementary strands of N4 DNA. We have used the HpaI restriction endonuclease fragments (Zivin et al., 1980) and single-stranded fragments of HhaI-digested N4 DNA as probes to determine the temporal appearance, localization and polarity of N4 transcripts. These experiments have allowed us to localize on the N4 genome the two previously characterized classes (early and middle RNAs) of N4 rifampicin-resistant RNAs (Vander Laan et al., 1977) and to detect a new class of N4 RNAs, which are synthesized late in infection. The synthesis of late N4 RNAs is rifampicin sensitive and, as we show in this paper, requires the activity of the Escherichia coli RNA polymerase.
AB - The strands of the four bacteriophage N4 DNA fragments derived by HhaI endonuclease digestion have been separated on agarose gels, and the eight fragments obtained have been related to the two complementary strands of N4 DNA. We have used the HpaI restriction endonuclease fragments (Zivin et al., 1980) and single-stranded fragments of HhaI-digested N4 DNA as probes to determine the temporal appearance, localization and polarity of N4 transcripts. These experiments have allowed us to localize on the N4 genome the two previously characterized classes (early and middle RNAs) of N4 rifampicin-resistant RNAs (Vander Laan et al., 1977) and to detect a new class of N4 RNAs, which are synthesized late in infection. The synthesis of late N4 RNAs is rifampicin sensitive and, as we show in this paper, requires the activity of the Escherichia coli RNA polymerase.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019839124&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(81)90246-1
DO - 10.1016/0022-2836(81)90246-1
M3 - Article
C2 - 6276570
AN - SCOPUS:0019839124
VL - 152
SP - 335
EP - 356
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 2
ER -