Transcriptional map of bacteriophage N4. Location and polarity of N4 RNAs

R. Zivin, W. Zehring, L. B. Rothman-Denes

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The strands of the four bacteriophage N4 DNA fragments derived by HhaI endonuclease digestion have been separated on agarose gels, and the eight fragments obtained have been related to the two complementary strands of N4 DNA. We have used the HpaI restriction endonuclease fragments (Zivin et al., 1980) and single-stranded fragments of HhaI-digested N4 DNA as probes to determine the temporal appearance, localization and polarity of N4 transcripts. These experiments have allowed us to localize on the N4 genome the two previously characterized classes (early and middle RNAs) of N4 rifampicin-resistant RNAs (Vander Laan et al., 1977) and to detect a new class of N4 RNAs, which are synthesized late in infection. The synthesis of late N4 RNAs is rifampicin sensitive and, as we show in this paper, requires the activity of the Escherichia coli RNA polymerase.

Original languageEnglish
Pages (from-to)335-356
Number of pages22
JournalJournal of Molecular Biology
Volume152
Issue number2
DOIs
StatePublished - Oct 25 1981

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Bacteriophage N4
RNA
Rifampin
DNA
DNA Restriction Enzymes
DNA Probes
DNA-Directed RNA Polymerases
Sepharose
Digestion
Gels
Genome
Escherichia coli
Infection

ASJC Scopus subject areas

  • Virology

Cite this

Transcriptional map of bacteriophage N4. Location and polarity of N4 RNAs. / Zivin, R.; Zehring, W.; Rothman-Denes, L. B.

In: Journal of Molecular Biology, Vol. 152, No. 2, 25.10.1981, p. 335-356.

Research output: Contribution to journalArticle

Zivin, R. ; Zehring, W. ; Rothman-Denes, L. B. / Transcriptional map of bacteriophage N4. Location and polarity of N4 RNAs. In: Journal of Molecular Biology. 1981 ; Vol. 152, No. 2. pp. 335-356.
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