Transcriptional co-expression and co-regulation of genes coding for components of the oxidative phosphorylation system

Corina van Waveren, Carlos T Moraes

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Background: The mitochondrial oxidative phosphorylation (OXPHOS) is critical for energy (ATP) production in eukaryotic cells. It was previously shown that genes coding for mitochondrial proteins involved in energy production co-express at the RNA level. Because the OXPHOS enzymes are multimeric complexes, we tested the hypothesis that genes coding for components of specific complexes are also co-regulated at the transcriptional level, and share common regulatory elements in their promoters. Results: We observed for the first time that, not only OXPHOS genes as a group co-express, but there is a co-expression of genes within each of the five OXPHOS enzyme complexes, showing a higher degree of complexity in gene co-regulation. In silico analysis of homologous promoter sequences in mammals identified the likely core promoter elements for most genes encoding OXPHOS subunits/assembly factors. The results included a significant abundance of previously identified sites (e.g. NRF1, NRF2, ERRA and YY1), as well as several sites that had not been previously detected. Although we identified patterns that correlated to OXPHOS gene expression, we did not detect an OXPHOS complex-specific arrangement of transcription factor binding sites within the core promoter that could explain the tight co-expression of these functionally related genes. Conclusion: This study mapped the core promoters of most OXPHOS related genes and provided an example of gene expression regulation based on the final protein arrangement within a linear metabolic pathway.

Original languageEnglish
Article number18
JournalBMC Genomics
Volume9
DOIs
StatePublished - Jan 14 2008

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Gene Components
Oxidative Phosphorylation
Genes
Gene Expression
Mitochondrial Proteins
Gene Expression Regulation
Eukaryotic Cells
Enzymes
Sequence Homology
Metabolic Networks and Pathways
Computer Simulation
Mammals
Transcription Factors
Adenosine Triphosphate
Binding Sites
RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Transcriptional co-expression and co-regulation of genes coding for components of the oxidative phosphorylation system. / van Waveren, Corina; Moraes, Carlos T.

In: BMC Genomics, Vol. 9, 18, 14.01.2008.

Research output: Contribution to journalArticle

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