Tracking clathrin coated pits with a multiple hypothesis based method

Liang Liang, Hongying Shen, Pietro De Camilli, James S. Duncan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

16 Scopus citations

Abstract

Cellular processes are crucial for cells to survive and function properly. To study their underlying mechanisms quantitatively with fluorescent live cell microscopy, it is necessary to track a large number of particles involved in these processes. In this paper, we present a method to automatically track particles, called clathrin coated pits (CCPs), which are formed in clathrin mediated endocytosis (CME). The tracking method is developed based on a MAP framework, and it consists of particle detection and trajectory estimation. To detect particles in 2D images and take account of Poisson noise, a Gaussian mixture model is fitted to image data, for which initial parameters are provided by a combination of image filtering and histogram based thresholding methods. A multiple hypothesis based algorithm is developed to estimate the trajectories based on detection data. To use the current knowledge about CCPs, their properties of motion and intensity are considered in our models. The tracking method is evaluated on synthetic data and real data, and experimental results show that it has high accuracy and is in good agreement with manual tracking.

Original languageEnglish (US)
Title of host publicationMedical Image Computing and Computer-Assisted Intervention, MICCAI2010 - 13th International Conference, Proceedings
Pages315-322
Number of pages8
EditionPART 2
DOIs
StatePublished - 2010
Externally publishedYes
Event13th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2010 - Beijing, China
Duration: Sep 20 2010Sep 24 2010

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
NumberPART 2
Volume6362 LNCS
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference13th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2010
Country/TerritoryChina
CityBeijing
Period9/20/109/24/10

ASJC Scopus subject areas

  • Theoretical Computer Science
  • Computer Science(all)

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