Tracheal mucosal blood flow responses to autonomic agonists

J. A. Barker, Alejandro D. Chediak, H. J. Baier, Adam Wanner

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In lightly anesthetized adult sheep, we determined tracheal mucosal blood flow (Q̇tr) by measuring the steady-state uptake of dimethyl ether from a tracheal chamber created by an endotracheal tube provided with two cuffs. Q̇tr normalized for carotid arterial pressure [Q̇tr(n)] was determined before and after the exposure of the tracheal mucosa to aerosolized phenylephrine (0.25-2.0 mg), isoproterenol (0.05-0.8 mg), and metacholine (2.5-20 mg). The same doses of metacholine were also administered during the intravenous infusion of vasopressin. The measurements were repeated after intravenous pretreatment with the respective antagonists phentolamine, propranolol, and atropine. Mean ± SE base-line Q̇tr(n) was 1.2 ± 0.1 ml · min-1 · mmHg-1 · 102. The autonomic antagonists had no effect on mean Q̇tr(n). Phenylephrine produced a dose-dependent decrease in mean Q̇tr(n) (-70% at the highest dose), which was blunted by phentolamine, and isoproterenol produced a dose-dependent increase in mean Q̇tr(n) (40% at the highest dose), which was blocked by propranolol. Metacholine failed to alter mean Q̇tr(n) even when Q̇tr was first decreased by vasopressin. We conclude that in lightly anesthetized adult sheep 1) base-line Q̇tr(n) is not under adrenergic or cholinergic control, 2) a locally administered α-adrenergic agonist decreases and β-adrenergic agonist increases Q̇tr(n) via specific receptor activation, and 3) a locally administered cholinergic muscarinic agonist has no effect on Q̇tr(n).

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalJournal of Applied Physiology
Volume65
Issue number2
StatePublished - Jan 1 1988

Fingerprint

Adrenergic Agonists
Phentolamine
Phenylephrine
Vasopressins
Isoproterenol
Propranolol
Sheep
Muscarinic Agonists
Atropine
Intravenous Infusions
Adrenergic Agents
Cholinergic Agents
Arterial Pressure
Mucous Membrane
dimethyl ether

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Tracheal mucosal blood flow responses to autonomic agonists. / Barker, J. A.; Chediak, Alejandro D.; Baier, H. J.; Wanner, Adam.

In: Journal of Applied Physiology, Vol. 65, No. 2, 01.01.1988, p. 829-834.

Research output: Contribution to journalArticle

@article{e01b1c86c88a4771ac451272f3673458,
title = "Tracheal mucosal blood flow responses to autonomic agonists",
abstract = "In lightly anesthetized adult sheep, we determined tracheal mucosal blood flow (Q̇tr) by measuring the steady-state uptake of dimethyl ether from a tracheal chamber created by an endotracheal tube provided with two cuffs. Q̇tr normalized for carotid arterial pressure [Q̇tr(n)] was determined before and after the exposure of the tracheal mucosa to aerosolized phenylephrine (0.25-2.0 mg), isoproterenol (0.05-0.8 mg), and metacholine (2.5-20 mg). The same doses of metacholine were also administered during the intravenous infusion of vasopressin. The measurements were repeated after intravenous pretreatment with the respective antagonists phentolamine, propranolol, and atropine. Mean ± SE base-line Q̇tr(n) was 1.2 ± 0.1 ml · min-1 · mmHg-1 · 102. The autonomic antagonists had no effect on mean Q̇tr(n). Phenylephrine produced a dose-dependent decrease in mean Q̇tr(n) (-70{\%} at the highest dose), which was blunted by phentolamine, and isoproterenol produced a dose-dependent increase in mean Q̇tr(n) (40{\%} at the highest dose), which was blocked by propranolol. Metacholine failed to alter mean Q̇tr(n) even when Q̇tr was first decreased by vasopressin. We conclude that in lightly anesthetized adult sheep 1) base-line Q̇tr(n) is not under adrenergic or cholinergic control, 2) a locally administered α-adrenergic agonist decreases and β-adrenergic agonist increases Q̇tr(n) via specific receptor activation, and 3) a locally administered cholinergic muscarinic agonist has no effect on Q̇tr(n).",
author = "Barker, {J. A.} and Chediak, {Alejandro D.} and Baier, {H. J.} and Adam Wanner",
year = "1988",
month = "1",
day = "1",
language = "English",
volume = "65",
pages = "829--834",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Tracheal mucosal blood flow responses to autonomic agonists

AU - Barker, J. A.

AU - Chediak, Alejandro D.

AU - Baier, H. J.

AU - Wanner, Adam

PY - 1988/1/1

Y1 - 1988/1/1

N2 - In lightly anesthetized adult sheep, we determined tracheal mucosal blood flow (Q̇tr) by measuring the steady-state uptake of dimethyl ether from a tracheal chamber created by an endotracheal tube provided with two cuffs. Q̇tr normalized for carotid arterial pressure [Q̇tr(n)] was determined before and after the exposure of the tracheal mucosa to aerosolized phenylephrine (0.25-2.0 mg), isoproterenol (0.05-0.8 mg), and metacholine (2.5-20 mg). The same doses of metacholine were also administered during the intravenous infusion of vasopressin. The measurements were repeated after intravenous pretreatment with the respective antagonists phentolamine, propranolol, and atropine. Mean ± SE base-line Q̇tr(n) was 1.2 ± 0.1 ml · min-1 · mmHg-1 · 102. The autonomic antagonists had no effect on mean Q̇tr(n). Phenylephrine produced a dose-dependent decrease in mean Q̇tr(n) (-70% at the highest dose), which was blunted by phentolamine, and isoproterenol produced a dose-dependent increase in mean Q̇tr(n) (40% at the highest dose), which was blocked by propranolol. Metacholine failed to alter mean Q̇tr(n) even when Q̇tr was first decreased by vasopressin. We conclude that in lightly anesthetized adult sheep 1) base-line Q̇tr(n) is not under adrenergic or cholinergic control, 2) a locally administered α-adrenergic agonist decreases and β-adrenergic agonist increases Q̇tr(n) via specific receptor activation, and 3) a locally administered cholinergic muscarinic agonist has no effect on Q̇tr(n).

AB - In lightly anesthetized adult sheep, we determined tracheal mucosal blood flow (Q̇tr) by measuring the steady-state uptake of dimethyl ether from a tracheal chamber created by an endotracheal tube provided with two cuffs. Q̇tr normalized for carotid arterial pressure [Q̇tr(n)] was determined before and after the exposure of the tracheal mucosa to aerosolized phenylephrine (0.25-2.0 mg), isoproterenol (0.05-0.8 mg), and metacholine (2.5-20 mg). The same doses of metacholine were also administered during the intravenous infusion of vasopressin. The measurements were repeated after intravenous pretreatment with the respective antagonists phentolamine, propranolol, and atropine. Mean ± SE base-line Q̇tr(n) was 1.2 ± 0.1 ml · min-1 · mmHg-1 · 102. The autonomic antagonists had no effect on mean Q̇tr(n). Phenylephrine produced a dose-dependent decrease in mean Q̇tr(n) (-70% at the highest dose), which was blunted by phentolamine, and isoproterenol produced a dose-dependent increase in mean Q̇tr(n) (40% at the highest dose), which was blocked by propranolol. Metacholine failed to alter mean Q̇tr(n) even when Q̇tr was first decreased by vasopressin. We conclude that in lightly anesthetized adult sheep 1) base-line Q̇tr(n) is not under adrenergic or cholinergic control, 2) a locally administered α-adrenergic agonist decreases and β-adrenergic agonist increases Q̇tr(n) via specific receptor activation, and 3) a locally administered cholinergic muscarinic agonist has no effect on Q̇tr(n).

UR - http://www.scopus.com/inward/record.url?scp=0023774327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023774327&partnerID=8YFLogxK

M3 - Article

C2 - 3049513

AN - SCOPUS:0023774327

VL - 65

SP - 829

EP - 834

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -