Toxicity of dimethyl sulfoxide on HeLa cells

Theodore Malinin, Vernon P. Perry

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

HeLa cells were exposed to dimethyl sulfoxide (DMSO) at +4 and at +22°C for periods of 30, 60, 90, and 120 min. At concentrations of DMSO below 15% there appeared to be no significant difference in the two temperatures of exposure as measured by confluency of cultures at the end of 96 hrs. Exposure at the lower temperature, however, did permit survival of cells in the higher concentrations of DMSO. The morphological effects of DMSO as examined with coverslip preparations stained with Maximow's hematoxylin eosin-azure II are also described. Pathological changes consisted of a typical mitotic figures, giant cells, and cells with ragged basophilie pseudopod-like projections. The number of abnormal cells increased with an increase in concentration of DMSO. The percentage of necrotic cells increased with an increase of DMSO concentration almost independent of the time of exposure or the time of subsequent culture.

Original languageEnglish
Pages (from-to)90-96
Number of pages7
JournalCryobiology
Volume4
Issue number2
StatePublished - Sep 1 1967
Externally publishedYes

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dimethyl sulfoxide
Dimethyl Sulfoxide
HeLa Cells
Toxicity
toxicity
cells
pseudopodia
Pseudopodia
Temperature
giant cells
Giant Cells
Hematoxylin
Eosine Yellowish-(YS)
cell viability
exposure duration
Cell Survival
temperature
Cell Count
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Malinin, T., & Perry, V. P. (1967). Toxicity of dimethyl sulfoxide on HeLa cells. Cryobiology, 4(2), 90-96.

Toxicity of dimethyl sulfoxide on HeLa cells. / Malinin, Theodore; Perry, Vernon P.

In: Cryobiology, Vol. 4, No. 2, 01.09.1967, p. 90-96.

Research output: Contribution to journalArticle

Malinin, T & Perry, VP 1967, 'Toxicity of dimethyl sulfoxide on HeLa cells', Cryobiology, vol. 4, no. 2, pp. 90-96.
Malinin T, Perry VP. Toxicity of dimethyl sulfoxide on HeLa cells. Cryobiology. 1967 Sep 1;4(2):90-96.
Malinin, Theodore ; Perry, Vernon P. / Toxicity of dimethyl sulfoxide on HeLa cells. In: Cryobiology. 1967 ; Vol. 4, No. 2. pp. 90-96.
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