Toxicity of aged gasoline exhaust particles to normal and diseased airway epithelia

Lisa Künzi, Manuel Krapf, Nancy Daher, Josef Dommen, Natalie Jeannet, Sarah Schneider, Stephen Platt, Jay G. Slowik, Nathalie Baumlin, Matthias A Salathe, André S H Prévôt, Markus Kalberer, Christof Strähl, Lutz Dümbgen, Constantinos Sioutas, Urs Baltensperger, Marianne Geiser

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Particulate matter (PM) pollution is a leading cause of premature death, particularly in those with pre-existing lung disease. A causative link between particle properties and adverse health effects remains unestablished mainly due to complex and variable physico-chemical PM parameters. Controlled laboratory experiments are required. Generating atmospherically realistic aerosols and performing cell-exposure studies at relevant particle-doses are challenging. Here we examine gasoline-exhaust particle toxicity from a Euro-5 passenger car in a uniquely realistic exposure scenario, combining a smog chamber simulating atmospheric ageing, an aerosol enrichment system varying particle number concentration independent of particle chemistry, and an aerosol deposition chamber physiologically delivering particles on air-liquid interface (ALI) cultures reproducing normal and susceptible health status. Gasoline-exhaust is an important PM source with largely unknown health effects. We investigated acute responses of fully-differentiated normal, distressed (antibiotics-treated) normal, and cystic fibrosis human bronchial epithelia (HBE), and a proliferating, single-cell type bronchial epithelial cell-line (BEAS-2B). We show that a single, short-term exposure to realistic doses of atmospherically-aged gasoline-exhaust particles impairs epithelial key-defence mechanisms, rendering it more vulnerable to subsequent hazards. We establish dose-response curves at realistic particle-concentration levels. Significant differences between cell models suggest the use of fully-differentiated HBE is most appropriate in future toxicity studies.

Original languageEnglish (US)
Article number11801
JournalScientific Reports
Volume5
DOIs
StatePublished - Jun 29 2015
Externally publishedYes

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Gasoline
Particulate Matter
Aerosols
Epithelium
Smog
Preexisting Condition Coverage
Premature Mortality
Health
Cystic Fibrosis
Lung Diseases
Health Status
Cause of Death
Epithelial Cells
Air
Anti-Bacterial Agents
Cell Line

ASJC Scopus subject areas

  • General

Cite this

Künzi, L., Krapf, M., Daher, N., Dommen, J., Jeannet, N., Schneider, S., ... Geiser, M. (2015). Toxicity of aged gasoline exhaust particles to normal and diseased airway epithelia. Scientific Reports, 5, [11801]. https://doi.org/10.1038/srep11801

Toxicity of aged gasoline exhaust particles to normal and diseased airway epithelia. / Künzi, Lisa; Krapf, Manuel; Daher, Nancy; Dommen, Josef; Jeannet, Natalie; Schneider, Sarah; Platt, Stephen; Slowik, Jay G.; Baumlin, Nathalie; Salathe, Matthias A; Prévôt, André S H; Kalberer, Markus; Strähl, Christof; Dümbgen, Lutz; Sioutas, Constantinos; Baltensperger, Urs; Geiser, Marianne.

In: Scientific Reports, Vol. 5, 11801, 29.06.2015.

Research output: Contribution to journalArticle

Künzi, L, Krapf, M, Daher, N, Dommen, J, Jeannet, N, Schneider, S, Platt, S, Slowik, JG, Baumlin, N, Salathe, MA, Prévôt, ASH, Kalberer, M, Strähl, C, Dümbgen, L, Sioutas, C, Baltensperger, U & Geiser, M 2015, 'Toxicity of aged gasoline exhaust particles to normal and diseased airway epithelia', Scientific Reports, vol. 5, 11801. https://doi.org/10.1038/srep11801
Künzi L, Krapf M, Daher N, Dommen J, Jeannet N, Schneider S et al. Toxicity of aged gasoline exhaust particles to normal and diseased airway epithelia. Scientific Reports. 2015 Jun 29;5. 11801. https://doi.org/10.1038/srep11801
Künzi, Lisa ; Krapf, Manuel ; Daher, Nancy ; Dommen, Josef ; Jeannet, Natalie ; Schneider, Sarah ; Platt, Stephen ; Slowik, Jay G. ; Baumlin, Nathalie ; Salathe, Matthias A ; Prévôt, André S H ; Kalberer, Markus ; Strähl, Christof ; Dümbgen, Lutz ; Sioutas, Constantinos ; Baltensperger, Urs ; Geiser, Marianne. / Toxicity of aged gasoline exhaust particles to normal and diseased airway epithelia. In: Scientific Reports. 2015 ; Vol. 5.
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