Abstract
Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and osteosarcoma, human breast and colon cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR, USA) and a recently synthesized mitochondrial oxygen probe, [PRE, (1″- pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in the blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochondrial distribution and morphology. In terms of application to biomedicine (for diagnostics, prognostics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnological applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | A.-C. Boccara |
| Pages | 322-332 |
| Number of pages | 11 |
| Volume | 5143 |
| State | Published - 2003 |
| Event | Novel Optical Instrumentation for Biomedical Applications - Munich, Germany Duration: Jun 24 2003 → Jun 25 2003 |
Other
| Other | Novel Optical Instrumentation for Biomedical Applications |
|---|---|
| Country | Germany |
| City | Munich |
| Period | 6/24/03 → 6/25/03 |
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Keywords
- Breast cancer cell
- Chlamydomonas
- Colon cancer cell
- Fluorescence spectral imaging
- Mitochondrial membrane potential probe
- Mitochondrial oxygen probe
- Osteosarcoma
- Photo acoustic spectroscopy
- Saccharomyces
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Towards Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy. / Kohen, Elli; Hirschberg, Joseph G.; Berry, John; Ozkutuk, Nuri; Ornek, Ceren; Monti, Marco; Leblanc, Roger; Schachtschabel, Dietrich O.; Haroon, Sumaira.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / A.-C. Boccara. Vol. 5143 2003. p. 322-332.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Towards Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy
AU - Kohen, Elli
AU - Hirschberg, Joseph G.
AU - Berry, John
AU - Ozkutuk, Nuri
AU - Ornek, Ceren
AU - Monti, Marco
AU - Leblanc, Roger
AU - Schachtschabel, Dietrich O.
AU - Haroon, Sumaira
PY - 2003
Y1 - 2003
N2 - Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and osteosarcoma, human breast and colon cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR, USA) and a recently synthesized mitochondrial oxygen probe, [PRE, (1″- pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in the blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochondrial distribution and morphology. In terms of application to biomedicine (for diagnostics, prognostics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnological applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.
AB - Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and osteosarcoma, human breast and colon cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR, USA) and a recently synthesized mitochondrial oxygen probe, [PRE, (1″- pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in the blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochondrial distribution and morphology. In terms of application to biomedicine (for diagnostics, prognostics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnological applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.
KW - Breast cancer cell
KW - Chlamydomonas
KW - Colon cancer cell
KW - Fluorescence spectral imaging
KW - Mitochondrial membrane potential probe
KW - Mitochondrial oxygen probe
KW - Osteosarcoma
KW - Photo acoustic spectroscopy
KW - Saccharomyces
UR - http://www.scopus.com/inward/record.url?scp=1342288563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1342288563&partnerID=8YFLogxK
M3 - Conference contribution
VL - 5143
SP - 322
EP - 332
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Boccara, A.-C.
ER -