Toward maximizing the success rates of human islet isolation: Influence of donor and isolation factors

Gaston M. Ponte, Antonello Pileggi, Shari Messinger, Angel Alejandro, Hirohito Ichii, David A. Baidal, Aisha Khan, Camillo Ricordi, John A. Goss, Rodolfo Alejandro

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

In order to make islet transplantation a therapeutic option for patients with diabetes there is an urgent need for more efficient islet cell processing to maximize islet recovery. Improved donor management, organ recovery techniques, implementation of more stringent donor criteria, and improved islet cell processing techniques may contribute to enhance organ utilization for transplantation. We have analyzed the effects of donor and islet processing factors on the success rate of human islet cell processing for transplantation performed at a single islet cell processing center. Islet isolation outcomes improved when vasopressors, and in particular pitressin, and steroids were used for the management of multiorgan donors. Higher islet yields were obtained from adult male donors, BMI >25 kg/m2, adequate glycemic control during hospital stay, and when the pancreas was retrieved by a local surgical team. Successful isolations were obtained in 58% of the cases when ≥ 4 donor criteria were met, and even higher success rates (69%) were observed when considering ≥ 5 criteria. Our data suggest that a sequential, integrated approach is highly desirable to improve the success rate of islet cell processing.

Original languageEnglish (US)
Pages (from-to)595-607
Number of pages13
JournalCell Transplantation
Volume16
Issue number6
DOIs
StatePublished - Jan 1 2007

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Keywords

  • Donor management
  • Islet isolation
  • Islet purification
  • Islet transplantation
  • Organ selection
  • Pancreas preservation
  • Pancreas recovery
  • Pancreatic islets

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

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