Toward high-throughput chip calorimetry by use of segmented-flow technology

A. Wolf, T. Hartmann, M. Bertolini, J. Schemberg, A. Grodrian, K. Lemke, T. Förster, E. Kessler, F. Hänschke, F. Mertens, R. Paus, J. Lerchner

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The adaptation of segmented-flow technology to flow-through calorimetry was demonstrated by different kinds of newly designed chip calorimeters useful for liquids and suspensions as well as for solid samples. In segmented-flow technology, sample material is suspended in aqueous segments of nano- or microliter volume and transported by a water-immiscible carrier liquid. The analysis of the signal dynamics given by segmented samples in flow led to optimal settings of flow rate and sample volume for maximal throughput. For 12 μL sample segments, a cycle time of 4 min could be achieved. The protection of the measuring chamber of the calorimeters against biofouling caused by the water-immiscible carrier liquid was verified for segmented bacterial samples. The unique possibility to measure solid and aggregated samples in flow-through was demonstrated by the investigation of human hair follicles and fibroblast spheroids.

Original languageEnglish (US)
Pages (from-to)172-183
Number of pages12
JournalThermochimica Acta
Volume603
DOIs
StatePublished - Mar 10 2015
Externally publishedYes

Keywords

  • Biofouling
  • Chip calorimetry
  • Hair follicles
  • Segmented-flow technology
  • Spheroids

ASJC Scopus subject areas

  • Instrumentation
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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    Wolf, A., Hartmann, T., Bertolini, M., Schemberg, J., Grodrian, A., Lemke, K., Förster, T., Kessler, E., Hänschke, F., Mertens, F., Paus, R., & Lerchner, J. (2015). Toward high-throughput chip calorimetry by use of segmented-flow technology. Thermochimica Acta, 603, 172-183. https://doi.org/10.1016/j.tca.2014.10.021