Topographic expression of p21WAF1/SDI1/CIP1, bcl2, and p53 is altered at the early stage of colorectal carcinogenesis

Jeong Hee Cho, Im Hwan Roe, Chang Young Lim, Dong Kook Park, Woo Ho Kim, Yong Il Kim

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We analyzed the expression of p21, bcl2, and p53 in normal and different pathologic mucosa of the human colorectum using immunohistochemistry and cold polymerase chain reaction-single strand conformation polymorphism. The topography of normal mucosa showed; bcl2 and p53 expression restricted to basal epithelial cells and p21 expressed only in superficial epithelial cells. This topographic expression was altered in hyperplastic polyps and adenomas. Hyperplastic polyps revealed absence of or weak bcl2 expression and strong p21 expression without topography. In adenomas, whereas bcl2 expression increased and extended to parabasal and superficial dysplastic epithelium, the increase of p21 expression was limited to surface dysplastic epithelium. p53 was weakly expressed throughout the full thickness of dysplastic epithelium. Bcl2 expression in adenomas was stronger than in carcinomas; p53 expression was converse and p21 expression was variable. In carcinomas, this topographic expression was largely abrogated but p53 mutation (36%) was more frequent than in adenomas (2%). In carcinomas, p21 and p53 expression correlated inversely, but there was no relationship with bcl2. These results suggest that there is precisely ordered topographic pattern of p21, bcl2, and wild p53 expression in normal colorectal cells, but this becomes disordered during the early stage of colorectal carcinogenesis.

Original languageEnglish (US)
Pages (from-to)667-674
Number of pages8
JournalJournal of Korean Medical Science
Volume15
Issue number6
DOIs
StatePublished - Dec 2000
Externally publishedYes

Keywords

  • Colorectal Carcinogenesis
  • Immunohistochemistry
  • PCR-SSCP
  • Topographic Expression
  • bcl2
  • p21
  • p53

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint

Dive into the research topics of 'Topographic expression of p21<sup>WAF1/SDI1/CIP1</sup>, bcl2, and p53 is altered at the early stage of colorectal carcinogenesis'. Together they form a unique fingerprint.

Cite this