Topical imipenem therapy of aminoglycoside-resistant pseudomonas keratitis in rabbits

M. R. Sawusch, Terrence O'Brien, J. Valentine, J. D. Dick, J. D. Gottsch

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We used a rabbit model of bacterial keratitis to assess in vivo efficacy of topical imipenem, a highly potent β-lactam antibiotic with an unusually broad spectrum of activity, including aminoglycoside-resistant Pseudomonas aeruginosa. Albino rabbits received intrastromal injections of 5 x 102 organisms of an aminoglycoside-resistant strain of P. aeruginosa. At five hours postinoculation, imipenem (5 mg/ml) therapy was initiated using one drop per 30 minutes for 12 hours. Corneal tissue was then excised for colony forming unit counts. Imipenem was highly effective in reducing colony forming unit counts to zero in comparison to 4.1 x 105 organisms for untreated controls. A second regimen beginning 24 hours postinoculation of one drop per hour for 24 hours was also successful in significantly reducing colony forming units vs controls (P < .05). These data suggest that topical imipenem may have clinical applicability in the treatment of P. aeruginosa keratitis.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalAmerican Journal of Ophthalmology
Volume106
Issue number1
StatePublished - Jan 1 1988
Externally publishedYes

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Keratitis
Imipenem
Aminoglycosides
Pseudomonas
Pseudomonas aeruginosa
Rabbits
Stem Cells
Lactams
Therapeutics
Anti-Bacterial Agents
Injections

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Sawusch, M. R., O'Brien, T., Valentine, J., Dick, J. D., & Gottsch, J. D. (1988). Topical imipenem therapy of aminoglycoside-resistant pseudomonas keratitis in rabbits. American Journal of Ophthalmology, 106(1), 77-81.

Topical imipenem therapy of aminoglycoside-resistant pseudomonas keratitis in rabbits. / Sawusch, M. R.; O'Brien, Terrence; Valentine, J.; Dick, J. D.; Gottsch, J. D.

In: American Journal of Ophthalmology, Vol. 106, No. 1, 01.01.1988, p. 77-81.

Research output: Contribution to journalArticle

Sawusch, MR, O'Brien, T, Valentine, J, Dick, JD & Gottsch, JD 1988, 'Topical imipenem therapy of aminoglycoside-resistant pseudomonas keratitis in rabbits', American Journal of Ophthalmology, vol. 106, no. 1, pp. 77-81.
Sawusch, M. R. ; O'Brien, Terrence ; Valentine, J. ; Dick, J. D. ; Gottsch, J. D. / Topical imipenem therapy of aminoglycoside-resistant pseudomonas keratitis in rabbits. In: American Journal of Ophthalmology. 1988 ; Vol. 106, No. 1. pp. 77-81.
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