Tonic activity of Galpha-gustducin regulates taste cell responsivity.

Tod R. Clapp; Kristina R. Trubey; Aurelie Vandenbeuch; Leslie M. Stone; Robert F. Margolskee; Nirupa Chaudhari; Sue C. Kinnamon

doi:10.1016/j.febslet.2008.10.007

Tonic activity of Galpha-gustducin regulates taste cell responsivity.

Tod R. Clapp, Kristina R. Trubey, Aurelie Vandenbeuch, Leslie M. Stone, Robert F. Margolskee, Nirupa Chaudhari, Sue C. Kinnamon

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The taste-selective G protein, α-gustducin (α-gus) is homologous to α-transducin and activates phosphodiesterase (PDE) in vitro. α-Gus-knockout mice are compromized to bitter, sweet and umami taste stimuli, suggesting a central role in taste transduction. Here, we suggest a different role for Gα-gus. In taste buds of α-gus-knockout mice, basal (unstimulated) cAMP levels are high compared to those of wild-type mice. Further, H-89, a cAMP-dependent protein kinase inhibitor, dramatically unmasks responses to the bitter tastant denatonium in gus-lineage cells of knockout mice. We propose that an important role of α-gus is to maintain cAMP levels tonically low to ensure adequate Ca²⁺ signaling.

Original language	English (US)
Pages (from-to)	3783-3787
Number of pages	5
Journal	FEBS letters
Volume	582
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2008.10.007
State	Published - Nov 12 2008
Externally published	Yes

Keywords

Calcium signaling
Phosphodiesterase
Protein kinase A
Taste transduction

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Genetics
Molecular Biology
Structural Biology

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title = "Tonic activity of Galpha-gustducin regulates taste cell responsivity.",

abstract = "The taste-selective G protein, α-gustducin (α-gus) is homologous to α-transducin and activates phosphodiesterase (PDE) in vitro. α-Gus-knockout mice are compromized to bitter, sweet and umami taste stimuli, suggesting a central role in taste transduction. Here, we suggest a different role for Gα-gus. In taste buds of α-gus-knockout mice, basal (unstimulated) cAMP levels are high compared to those of wild-type mice. Further, H-89, a cAMP-dependent protein kinase inhibitor, dramatically unmasks responses to the bitter tastant denatonium in gus-lineage cells of knockout mice. We propose that an important role of α-gus is to maintain cAMP levels tonically low to ensure adequate Ca2+ signaling.",

keywords = "Calcium signaling, Phosphodiesterase, Protein kinase A, Taste transduction",

author = "Clapp, {Tod R.} and Trubey, {Kristina R.} and Aurelie Vandenbeuch and Stone, {Leslie M.} and Margolskee, {Robert F.} and Nirupa Chaudhari and Kinnamon, {Sue C.}",

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T1 - Tonic activity of Galpha-gustducin regulates taste cell responsivity.

AU - Clapp, Tod R.

AU - Trubey, Kristina R.

AU - Vandenbeuch, Aurelie

AU - Stone, Leslie M.

AU - Margolskee, Robert F.

AU - Chaudhari, Nirupa

AU - Kinnamon, Sue C.

PY - 2008/11/12

Y1 - 2008/11/12

N2 - The taste-selective G protein, α-gustducin (α-gus) is homologous to α-transducin and activates phosphodiesterase (PDE) in vitro. α-Gus-knockout mice are compromized to bitter, sweet and umami taste stimuli, suggesting a central role in taste transduction. Here, we suggest a different role for Gα-gus. In taste buds of α-gus-knockout mice, basal (unstimulated) cAMP levels are high compared to those of wild-type mice. Further, H-89, a cAMP-dependent protein kinase inhibitor, dramatically unmasks responses to the bitter tastant denatonium in gus-lineage cells of knockout mice. We propose that an important role of α-gus is to maintain cAMP levels tonically low to ensure adequate Ca2+ signaling.

AB - The taste-selective G protein, α-gustducin (α-gus) is homologous to α-transducin and activates phosphodiesterase (PDE) in vitro. α-Gus-knockout mice are compromized to bitter, sweet and umami taste stimuli, suggesting a central role in taste transduction. Here, we suggest a different role for Gα-gus. In taste buds of α-gus-knockout mice, basal (unstimulated) cAMP levels are high compared to those of wild-type mice. Further, H-89, a cAMP-dependent protein kinase inhibitor, dramatically unmasks responses to the bitter tastant denatonium in gus-lineage cells of knockout mice. We propose that an important role of α-gus is to maintain cAMP levels tonically low to ensure adequate Ca2+ signaling.

KW - Calcium signaling

KW - Phosphodiesterase

KW - Protein kinase A

KW - Taste transduction

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