Tonic activity of Gα-gustducin regulates taste cell responsivity

Tod R. Clapp, Kristina R. Trubey, Aurelie Vandenbeuch, Leslie M. Stone, Robert F. Margolskee, Nirupa Chaudhari, Sue C. Kinnamon

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The taste-selective G protein, α-gustducin (α-gus) is homologous to α-transducin and activates phosphodiesterase (PDE) in vitro. α-Gus-knockout mice are compromized to bitter, sweet and umami taste stimuli, suggesting a central role in taste transduction. Here, we suggest a different role for Gα-gus. In taste buds of α-gus-knockout mice, basal (unstimulated) cAMP levels are high compared to those of wild-type mice. Further, H-89, a cAMP-dependent protein kinase inhibitor, dramatically unmasks responses to the bitter tastant denatonium in gus-lineage cells of knockout mice. We propose that an important role of α-gus is to maintain cAMP levels tonically low to ensure adequate Ca2+ signaling.

Original languageEnglish
Pages (from-to)3783-3787
Number of pages5
JournalFEBS Letters
Volume582
Issue number27
DOIs
StatePublished - Nov 12 2008
Externally publishedYes

Fingerprint

Transducin
Phosphoric Diester Hydrolases
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
GTP-Binding Proteins
Knockout Mice
Taste Buds
gustducin
N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide

Keywords

  • Calcium signaling
  • Phosphodiesterase
  • Protein kinase A
  • Taste transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Clapp, T. R., Trubey, K. R., Vandenbeuch, A., Stone, L. M., Margolskee, R. F., Chaudhari, N., & Kinnamon, S. C. (2008). Tonic activity of Gα-gustducin regulates taste cell responsivity. FEBS Letters, 582(27), 3783-3787. https://doi.org/10.1016/j.febslet.2008.10.007

Tonic activity of Gα-gustducin regulates taste cell responsivity. / Clapp, Tod R.; Trubey, Kristina R.; Vandenbeuch, Aurelie; Stone, Leslie M.; Margolskee, Robert F.; Chaudhari, Nirupa; Kinnamon, Sue C.

In: FEBS Letters, Vol. 582, No. 27, 12.11.2008, p. 3783-3787.

Research output: Contribution to journalArticle

Clapp, TR, Trubey, KR, Vandenbeuch, A, Stone, LM, Margolskee, RF, Chaudhari, N & Kinnamon, SC 2008, 'Tonic activity of Gα-gustducin regulates taste cell responsivity', FEBS Letters, vol. 582, no. 27, pp. 3783-3787. https://doi.org/10.1016/j.febslet.2008.10.007
Clapp TR, Trubey KR, Vandenbeuch A, Stone LM, Margolskee RF, Chaudhari N et al. Tonic activity of Gα-gustducin regulates taste cell responsivity. FEBS Letters. 2008 Nov 12;582(27):3783-3787. https://doi.org/10.1016/j.febslet.2008.10.007
Clapp, Tod R. ; Trubey, Kristina R. ; Vandenbeuch, Aurelie ; Stone, Leslie M. ; Margolskee, Robert F. ; Chaudhari, Nirupa ; Kinnamon, Sue C. / Tonic activity of Gα-gustducin regulates taste cell responsivity. In: FEBS Letters. 2008 ; Vol. 582, No. 27. pp. 3783-3787.
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