Tomosyn is expressed in β-cells and negatively regulates insulin exocytosis

Wei Zhang, Lena Lilja, Slavena A. Mandic, Jesper Gromada, Kamille Smidt, Juliette Janson, Yoshimi Takai, Christina Bark, Per Olof Berggren, Björn Meister

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Tomosyn, a syntaxin-binding protein, is capable of dissociating mammalian homolog of the Caenorhabditis elegans unc-18 gene from syntaxin and is involved in the regulation of exocytosis. We have investigated the expression, cellular localization, and functional role of tomosyn in pancreatic β-cells. Western blotting revealed a 130-kDa protein corresponding to tomosyn in insulin-secreting β-cell lines. RT-PCR amplification showed that b-, m-, and s-tomosyn isoform mRNAs are expressed in β-cell lines and rat pancreatic islets. Immunohistochemistry revealed punctate tomosyn immunoreactivity in the cytoplasm of insulin-, glucagon-, pancreatic polypeptide-, and somatostatin-containing islet cells. Syntaxin 1 coimmunoprecipitated with tomosyn in extracts of insulin-secreting cells. Overexpression of m-tomosyn in mouse β-cells significantly decreased exocytosis, whereas inhibition of tomosyn expression by small interfering RNA increased exocytosis. Hence, in the pancreatic β-cell, tomosyn negatively regulates insulin exocytosis.

Original languageEnglish
Pages (from-to)574-581
Number of pages8
JournalDiabetes
Volume55
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

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Exocytosis
Insulin
Qa-SNARE Proteins
Insulin-Secreting Cells
Islets of Langerhans
Syntaxin 1
RNA Isoforms
Pancreatic Polypeptide
Cell Line
Caenorhabditis elegans
Somatostatin
Glucagon
Small Interfering RNA
Carrier Proteins
Cytoplasm
Western Blotting
Immunohistochemistry
Polymerase Chain Reaction
Genes
Proteins

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Zhang, W., Lilja, L., Mandic, S. A., Gromada, J., Smidt, K., Janson, J., ... Meister, B. (2006). Tomosyn is expressed in β-cells and negatively regulates insulin exocytosis. Diabetes, 55(3), 574-581. https://doi.org/10.1002/sia.2299

Tomosyn is expressed in β-cells and negatively regulates insulin exocytosis. / Zhang, Wei; Lilja, Lena; Mandic, Slavena A.; Gromada, Jesper; Smidt, Kamille; Janson, Juliette; Takai, Yoshimi; Bark, Christina; Berggren, Per Olof; Meister, Björn.

In: Diabetes, Vol. 55, No. 3, 01.03.2006, p. 574-581.

Research output: Contribution to journalArticle

Zhang, W, Lilja, L, Mandic, SA, Gromada, J, Smidt, K, Janson, J, Takai, Y, Bark, C, Berggren, PO & Meister, B 2006, 'Tomosyn is expressed in β-cells and negatively regulates insulin exocytosis', Diabetes, vol. 55, no. 3, pp. 574-581. https://doi.org/10.1002/sia.2299
Zhang W, Lilja L, Mandic SA, Gromada J, Smidt K, Janson J et al. Tomosyn is expressed in β-cells and negatively regulates insulin exocytosis. Diabetes. 2006 Mar 1;55(3):574-581. https://doi.org/10.1002/sia.2299
Zhang, Wei ; Lilja, Lena ; Mandic, Slavena A. ; Gromada, Jesper ; Smidt, Kamille ; Janson, Juliette ; Takai, Yoshimi ; Bark, Christina ; Berggren, Per Olof ; Meister, Björn. / Tomosyn is expressed in β-cells and negatively regulates insulin exocytosis. In: Diabetes. 2006 ; Vol. 55, No. 3. pp. 574-581.
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