Toll, a muscle cell surface molecule, locally inhibits synaptic initiation of the RP3 motoneuron growth cone in Drosophila

Demian Rose, Xiaomao Zhu, Hiroyuki Kose, Bao Hoang, John Cho, Akira Chiba

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Toll, a transmembrane molecule with extracellular leucine-rich repeats, is dynamically expressed by the Drosophila embryonic musculature. Growth cones of RP3 and other motoneurons normally grow past Toll-positive muscle cells and innervate more distal muscle cells, which have down-regulated their Toll expression. In this study, we show that reciprocal genetic manipulations of Toll proteins can produce reciprocal RP3 phenotypes. In Toll null mutants, the RP3 growth cone sometimes innervates incorrect muscle cells, including those that are normally Toll-positive. In contrast, heterochronic misexpression of Toll in the musculature leads to the same growth cone reaching its correct target region but delaying synaptic initiation. We propose that Toll acts locally to inhibit synaptogenesis of specific motoneuron growth cones and that both temporal and spatial control of Toll expression is crucial for its role in development.

Original languageEnglish
Pages (from-to)1561-1571
Number of pages11
JournalDevelopment
Volume124
Issue number8
StatePublished - Apr 1 1997
Externally publishedYes

Fingerprint

Growth Cones
Motor Neurons
Muscle Cells
Drosophila
Leucine
Phenotype
Proteins

Keywords

  • Drosophila
  • Growth cone
  • Leucine-rich repeat
  • Misexpression
  • Neuromuscular
  • Synaptogenesis
  • Target recognition
  • Temporal control
  • Toll

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Toll, a muscle cell surface molecule, locally inhibits synaptic initiation of the RP3 motoneuron growth cone in Drosophila. / Rose, Demian; Zhu, Xiaomao; Kose, Hiroyuki; Hoang, Bao; Cho, John; Chiba, Akira.

In: Development, Vol. 124, No. 8, 01.04.1997, p. 1561-1571.

Research output: Contribution to journalArticle

Rose, Demian ; Zhu, Xiaomao ; Kose, Hiroyuki ; Hoang, Bao ; Cho, John ; Chiba, Akira. / Toll, a muscle cell surface molecule, locally inhibits synaptic initiation of the RP3 motoneuron growth cone in Drosophila. In: Development. 1997 ; Vol. 124, No. 8. pp. 1561-1571.
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AU - Cho, John

AU - Chiba, Akira

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