TNFα is involved in neuropathic pain induced by nucleoside reverse transcriptase inhibitor in rats

Xuexing Zheng, Handong Ouyang, Shue Liu, Marina Mata, David J. Fink, Shuanglin Hao

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

In patients with HIV/AIDS, neuropathic pain is a common neurological complication. Infection with the HIV itself may lead to neuropathic pain, and painful symptoms are enhanced when patients are treated with nucleoside reverse transcriptase inhibitors (NRTIs). The mechanisms by which NRTIs contribute to the development of neuropathic pain are not known. In the current studies, we tested the role of TNFα in antiretroviral drug-induced neuropathic pain. We administered 2',3'-dideoxycytidine (ddC, one of the NRTIs) systemically to induce mechanical allodynia. We found that ddC induced overexpression of both mRNA and proteins of GFAP and TNFα in the spinal dorsal horn. TNFα was colocalized with GFAP in the spinal dorsal horn and with NeuN in the DRG. Knockdown of TNFα with siRNA blocked the mechanical allodynia induced by ddC. Intrathecal administration of glial inhibitor or recombinant TNF soluble receptor, reversed mechanical allodynia induced by ddC. These results suggest that TNFα is involved in NRTI-induced neuropathic pain.

Original languageEnglish (US)
Pages (from-to)1668-1676
Number of pages9
JournalBrain, Behavior, and Immunity
Volume25
Issue number8
DOIs
StatePublished - Nov 1 2011

Keywords

  • DdC
  • HIV
  • Neuropathic pain
  • TNFα

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

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