Titration curve of a proteolytic enzyme

Mario A. Marini, Christian Wunsch

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A procedure is presented for the determination of the hydrogen ion equilibria of the proteolytic enzyme, α-chymotrypsin. Estimation of the extent of autolysis has been made from the excess amino groups present in the formol titration, by the rapid reversal technique of Steinhardt and Zaiser (16), and by retitration.

Original languageEnglish (US)
Pages (from-to)534-542
Number of pages9
JournalAnalytical Biochemistry
Volume6
Issue number6
DOIs
StatePublished - Dec 1963
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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