Tissue transport and initial processing cryostat preparation of slides

Michael P. McLeod, Sonal Choudhary, Katlein França, Keyvan Nouri

Research output: Chapter in Book/Report/Conference proceedingChapter


The major goal during tissue transport and initial processing is to maintain specimen orientation. Once the specimen is removed from the surgical site and hemostasis is achieved, the Mohs map can be drawn. Then the specimen is transported to the histopathology laboratory. The specimen should be cut into as few sections as possible in order to avoid orientation error, while still producing high-quality slides. Colored ink is used to assist in maintaining orientation. Optimum cutting temperature (OCT) is used to facilitate specimen adhesion to the microtome chuck. Following microtome sectioning, the specimen is stained, most commonly with hematoxylin and eosin. After application of the coverslip, the slides are ready to be analyzed by the Mohs surgeon.

Original languageEnglish (US)
Title of host publicationMohs Micrographic Surgery
PublisherSpringer-Verlag London Ltd
Number of pages5
ISBN (Print)9781447121527, 1447121511, 9781447121510
StatePublished - Jun 1 2012


  • Hematoxylin and eosin
  • Mohs map
  • Mohs micrographic surgery initial processing
  • Tissue transport

ASJC Scopus subject areas

  • Medicine(all)


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