Tissue transport and initial processing cryostat preparation of slides

Michael P. McLeod, Sonal Choudhary, Katlein França, Keyvan Nouri

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The major goal during tissue transport and initial processing is to maintain specimen orientation. Once the specimen is removed from the surgical site and hemostasis is achieved, the Mohs map can be drawn. Then the specimen is transported to the histopathology laboratory. The specimen should be cut into as few sections as possible in order to avoid orientation error, while still producing high-quality slides. Colored ink is used to assist in maintaining orientation. Optimum cutting temperature (OCT) is used to facilitate specimen adhesion to the microtome chuck. Following microtome sectioning, the specimen is stained, most commonly with hematoxylin and eosin. After application of the coverslip, the slides are ready to be analyzed by the Mohs surgeon.

Original languageEnglish (US)
Title of host publicationMohs Micrographic Surgery
PublisherSpringer-Verlag London Ltd
Pages117-121
Number of pages5
Volume9781447121527
ISBN (Print)9781447121527, 1447121511, 9781447121510
DOIs
StatePublished - Jun 1 2012

Fingerprint

Ink
Hematoxylin
Eosine Yellowish-(YS)
Hemostasis
Temperature
Surgeons

Keywords

  • Hematoxylin and eosin
  • Mohs map
  • Mohs micrographic surgery initial processing
  • Tissue transport

ASJC Scopus subject areas

  • Medicine(all)

Cite this

McLeod, M. P., Choudhary, S., França, K., & Nouri, K. (2012). Tissue transport and initial processing cryostat preparation of slides. In Mohs Micrographic Surgery (Vol. 9781447121527, pp. 117-121). Springer-Verlag London Ltd. https://doi.org/10.1007/978-1-4471-2152-7_12

Tissue transport and initial processing cryostat preparation of slides. / McLeod, Michael P.; Choudhary, Sonal; França, Katlein; Nouri, Keyvan.

Mohs Micrographic Surgery. Vol. 9781447121527 Springer-Verlag London Ltd, 2012. p. 117-121.

Research output: Chapter in Book/Report/Conference proceedingChapter

McLeod, MP, Choudhary, S, França, K & Nouri, K 2012, Tissue transport and initial processing cryostat preparation of slides. in Mohs Micrographic Surgery. vol. 9781447121527, Springer-Verlag London Ltd, pp. 117-121. https://doi.org/10.1007/978-1-4471-2152-7_12
McLeod MP, Choudhary S, França K, Nouri K. Tissue transport and initial processing cryostat preparation of slides. In Mohs Micrographic Surgery. Vol. 9781447121527. Springer-Verlag London Ltd. 2012. p. 117-121 https://doi.org/10.1007/978-1-4471-2152-7_12
McLeod, Michael P. ; Choudhary, Sonal ; França, Katlein ; Nouri, Keyvan. / Tissue transport and initial processing cryostat preparation of slides. Mohs Micrographic Surgery. Vol. 9781447121527 Springer-Verlag London Ltd, 2012. pp. 117-121
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