Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype

Yuanyuan Zhang, Yujiang He, Shantaram Bharadwaj, Nevin Hammam, Kristen Carnagey, Regina Myers, Anthony Atala, Mark Van Dyke

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

Recent studies have shown that extracellular matrix (ECM) substitutes can have a dramatic impact on cell growth, differentiation and function. However, these ECMs are often applied generically and have yet to be developed for specific cell types. In this study, we developed tissue-specific ECM-based coating substrates for skin, skeletal muscle and liver cell cultures. Cellular components were removed from adult skin, skeletal muscle, and liver tissues, and the resulting acellular matrices were homogenized and dissolved. The ECM solutions were used to coat culture dishes. Tissue matched and non-tissue matched cell types were grown on these coatings to assess adhesion, proliferation, maintenance of phenotype and cell function at several time points. Each cell type showed better proliferation and differentiation in cultures containing ECM from their tissue of origin. Although subtle compositional differences in the three ECM types were not investigated in this study, these results suggest that tissue-specific ECMs provide a culture microenvironment that is similar to the in vivo environment when used as coating substrates, and this new culture technique has the potential for use in drug development and the development of cell-based therapies.

Original languageEnglish (US)
Pages (from-to)4021-4028
Number of pages8
JournalBiomaterials
Volume30
Issue number23-24
DOIs
StatePublished - Aug 1 2009

Keywords

  • Adhesion
  • Cell culture
  • Cell proliferation
  • ECM (extracellular matrix)

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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    Zhang, Y., He, Y., Bharadwaj, S., Hammam, N., Carnagey, K., Myers, R., Atala, A., & Van Dyke, M. (2009). Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype. Biomaterials, 30(23-24), 4021-4028. https://doi.org/10.1016/j.biomaterials.2009.04.005